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SKU
MB21201
T7 Endonuclease I
T7 Endonuclease I is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer.
Availability
Ready to Ship
Dry Ice
T7 Endonuclease I is an ultrapure recombinant enzyme purified from Escherichia coli. This endonuclease is the product of phage T7 gene 3. T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double stranded DNA. The cleavage site is the first, second or third phosphodiester bond that is 5´ to the mismatch. The enzyme has a variety of applications, including: (1) resolving four-way junction or branched DNA; (2) detection or cleavage of heteroduplex and nicked DNA; (3) random cleavage of linear DNA for shot-gun cloning.
Applications:
– Resolving four-way junction or branched DNA
– Detection or cleavage of heteroduplex and nicked DNA
– Random cleavage of linear DNA for shot-gun cloning
Applications:
– Resolving four-way junction or branched DNA
– Detection or cleavage of heteroduplex and nicked DNA
– Random cleavage of linear DNA for shot-gun cloning
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | Mismatch nicking |
Product Category | Nucleases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
– T7 Endonuclease I (250 U)
– Reaction buffer (10x)
– Reaction buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | Mismatch nicking |
Product Category | Nucleases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | Mismatch nicking |
Product Category | Nucleases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
CoA
Certificate of Analysis