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SKU
MB21201

T7 Endonuclease I

Storage Conditions:
-85 °C to -15 °C
T7 Endonuclease I is an ultrapure recombinant enzyme purified from Escherichia coli supplied with an optimized 10× Reaction Buffer.
Availability
Ready to Ship
Dry Ice
Unit Size
250 U
€92.00
T7 Endonuclease I is an ultrapure recombinant enzyme purified from Escherichia coli. This endonuclease is the product of phage T7 gene 3. T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double stranded DNA. The cleavage site is the first, second or third phosphodiester bond that is 5´ to the mismatch. The enzyme has a variety of applications, including: (1) resolving four-way junction or branched DNA; (2) detection or cleavage of heteroduplex and nicked DNA; (3) random cleavage of linear DNA for shot-gun cloning.
Applications:
– Resolving four-way junction or branched DNA
– Detection or cleavage of heteroduplex and nicked DNA
– Random cleavage of linear DNA for shot-gun cloning
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Mismatch nicking
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 10 U/μL
– T7 Endonuclease I (250 U)
– Reaction buffer (10x)
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Mismatch nicking
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 10 U/μL
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Mismatch nicking
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 10 U/μL

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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