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SKU
MB21301

Endonuclease V (E. coli)

Storage Conditions:
-85 °C to -15 °C
Endonuclease V is a repair enzyme from Escherichia coli, expressed in the recombinant form in the same host, which recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA.
Availability
Ready to Ship
Dry Ice
Unit Size
250 U
€103.00
Endonuclease V is a DNA repair enzyme from Escherichia coli, expressed in the recombinant form in the same host, which recognizes deaminated bases. Specifically, the enzyme recognizes deoxyinosines in DNA strands (double or single-stranded), obtained by deamination of deoxyadenosines. Endonuclease V also recognizes DNA containing abasic sites or urea sites, base mismatches, harpins or loops. Endonuclease V, also termed deoxyinosine 3´ endonuclease, catalyses the cleavage of the second phosphodiester bond 3´ to the mismatch of deoxyinosine, leaving a nick with 3´-OH and 5´-phosphate. The enzyme has a variety of applications, including: cleavage of oligonucleotides containing deoxyinosines and base mismatches cleavage. Endonuclease V has also affinity for oligonucleotides containing base mismatches.
Applications:
– DNA mismatch cleavage
– Affinity for oligonucleotides containing deoxyinosines
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Mismatch nicking
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 10 U/μL
– Endonuclease V (250 U)
– Reaction buffer (10x)
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Mismatch nicking
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 10 U/μL
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Activity Mismatch nicking
Product Category Nucleases
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 10 U/μL

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