Endonuclease V (E. coli) (MB21301)

Refrigerated package

91,00

250 U

91,00

Description: Endonuclease V is a repair enzyme from Escherichia coli, expressed in the recombinant form in the same host, which recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA. Endonuclease V, also termed deoxyinosine 3´ endonuclease, recognizes DNA containing deoxyinosines (paired or not) on double-stranded DNA, single-stranded DNA with deoxyinosines and to a lesser degree, DNA containing abasic sites (ap) or urea, base mismatches, insertion/deletion mismatches, hairpin or unpaired loops, flaps and pseudo-Y structures.

Features:
– >95% pure as judged by SDS-PAGE
– Supplied with an optimized reaction buffer for efficient activity

Applications:
– DNA mismatch cleavage
– Affinity for oligonucleotides containing deoxyinosines

Specifications:

Enzyme concentration:10 U/μL
Source:Recombinant E. coli strain
Optimal reaction temperature:37 °C
Heat inactivation:65 °C for 20 min
Storage conditions:Store at -20 °C
Shipping conditions:Shipped at dry ice

Components:
– Endonuclease V (250 U)
– Reaction buffer (10x)

Product Brochure EN
MSDS PT
MSDS EN

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