T5 Exonuclease (MB43301)

Refrigerated package

59,00

1000 U

59,00

Description: T5 Exonuclease is a typical DNA nuclease that functions in the 5´ to 3´ direction and attacks both single and double-stranded DNA. The enzyme substrate is the 5´-termini of a DNA molecule in the context of single or double-stranded nucleic acids as well as gaps and nicks of linear or circular double-stranded DNA. However, T5 Exonuclease does not degrade supercoiled double-stranded DNA. Thus, the enzyme may be used to remove denatured DNA generated during the alkaline-lysis protocols for plasmid purification or linear and circular DNA from supercoiled plasmid preps. It may also be used to remove incomplete ligation products from ligated circular double-stranded DNA.

Features:
– Substrate specific DNA nuclease: double-stranded DNA specific exonuclease and single-stranded DNA endonuclease
– Catalyses the degradation of linear or nicked double-stranded DNA in a 5’ to 3’ direction

Applications:
– Degradation of linear ssDNA, dsDNA circular or nicked plasmid DNA
– No degradation of supercoiled plasmid DNA
– Removal of denatured DNA generated during alkaline-lysis plasmid purification procedures
– Removal of incomplete ligation products from ligated circular double-stranded DNA

Specifications:

Enzyme concentration:10 U/μL
Source:Recombinant E. coli strain
Optimal reaction temperature:37 °C
Heat inactivation:No
Storage conditions:Store at -20 °C
Shipping conditions:Shipped at dry ice

Components:
– T5 Exonuclease (10 U/μL)
– Reaction Buffer (10x)

Product Brochure EN
Certificate of Analysis NM031

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