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SKU
MB43301
T5 Exonuclease
T5 Exonuclease is a typical DNA nuclease that functions in the 5´ to 3´ direction and attacks both single and double-stranded DNA.
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Dry Ice
T5 Exonuclease is a typical DNA nuclease that functions in the 5´ to 3´ direction and attacks both single and double-stranded DNA. The enzyme substrate is the 5´-termini of a DNA molecule in the context of single or double-stranded nucleic acids as well as gaps and nicks of linear or circular double-stranded DNA. However, T5 Exonuclease does not degrade supercoiled double-stranded DNA. Thus, the enzyme may be used to remove denatured DNA generated during the alkaline-lysis protocols for plasmid purification or linear and circular DNA from supercoiled plasmid preps. It may also be used to remove incomplete ligation products from ligated circular double-stranded DNA.
Applications:
– Degradation of linear ssDNA, dsDNA circular or nicked plasmid DNA
– No degradation of supercoiled plasmid DNA
– Removal of denatured DNA generated during alkaline-lysis plasmid purification procedures
– Removal of incomplete ligation products from ligated circular double-stranded DNA
Applications:
– Degradation of linear ssDNA, dsDNA circular or nicked plasmid DNA
– No degradation of supercoiled plasmid DNA
– Removal of denatured DNA generated during alkaline-lysis plasmid purification procedures
– Removal of incomplete ligation products from ligated circular double-stranded DNA
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 5′ → 3′ DNA nuclease |
Product Category | Nucleases |
Reaction Temperature | 37 °C |
Heat Inactivation | No |
Concentration | 10 U/μL |
– T5 Exonuclease (10 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 5′ → 3′ DNA nuclease |
Product Category | Nucleases |
Reaction Temperature | 37 °C |
Heat Inactivation | No |
Concentration | 10 U/μL |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 5′ → 3′ DNA nuclease |
Product Category | Nucleases |
Reaction Temperature | 37 °C |
Heat Inactivation | No |
Concentration | 10 U/μL |
CoA
Certificate of Analysis