T4 Polynucleotide Kinase (T4 PNK) (MB00801)

Refrigerated package

73,00

500 U

73,00

Description: T4 Polynucleotide Kinase catalyses the transfer of the terminal phosphate of ATP to 5′-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3′-mononucleotides. In the presence of ADP, it will also catalyse the exchange of 5′-terminal phosphate groups and ATP. This enzyme also possesses a 3′-phosphatase and 2′, 3′ cyclic phosphodiesterase activity.

Features:
– Free from detectable exonuclease, endonuclease and ribonuclease activities
– Supplied with an optimized reaction buffer for efficient activity
– Supplied with a dilution buffer to optimize levels of incorporation

Applications:
– End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
– 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
– Removal of 3´-phosphoryl groups

Specifications:

Enzyme concentration: 30 U/μL
Source: Recombinant E. coli strain
Optimal reaction temperature: 37 °C
Heat inactivation: 65 °C for 10 min
Storage conditions: Store at -20 °C
Shipping conditions: Shipped at dry ice

Components:
– T4 Polynucleotide Kinase (500 U)
– Reaction buffer (10x)
– Dilution buffer

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis YO021

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