Description: Alkaline Phosphatase is suitable for removal of terminal monoesterified phosphates from deoxyribo-oligonucleotides. Used for the removal of single phosphate groups from 5´-ends of linear vectors to prevent re-circularization during cloning or to dephosphorylate DNA prior to kinase labelling protocols.
Features:
– Free from detectable nonspecific nuclease, endonuclease, RNase and DNase activities
– Very stable enzyme
– Supplied with an optimized reaction buffer containing Zn2+ for efficient activity
Applications:
– Dephosphorylation of DNA
– Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis
Specifications:
| Enzyme concentration: | 0.5 U/μL |
| Source: | Recombinant E. coli strain |
| Optimal reaction temperature: | 37 °C |
| Denaturing conditions: |
Temporarily inactivated after incubation at 100 °C in the presence of a chelating reagent (the enzyme recovers activity when returned to room temperature). Phenol extraction or DNA purification using NZYTech ́s NZYGelpure is, therefore, recommended.
|
| Storage conditions: | Store at -20 °C |
| Shipping conditions: | Shipped at dry ice |
Components:
– Alkaline Phosphatase (200 U)
– Reaction buffer (10x)
