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SKU
MB01801
Alkaline Phosphatase (E. coli)
Alkaline Phosphatase is suitable for removal of terminal monoesterified phosphates from deoxyribo-oligonucleotides.
Availability
Ready to Ship
Dry Ice
Alkaline Phosphatase (E. coli) non-specifically catalyses the removal of most phosphomonoester bonds from the 5' and 3' termini of DNA and RNA without degrading diphosphate or triphosphate linkages. The enzyme is suitable for removal of terminal monoesterified phosphates from deoxyribo-oligonucleotides. The enzyme is generally used for the removal of single phosphate groups from 5´-ends of linear vectors to prevent re-circularization during cloning or to dephosphorylate DNA prior to kinase labelling protocols.
Applications:
– Dephosphorylation of DNA
– Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis
Applications:
– Dephosphorylation of DNA
– Treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | 5'-dephosphorylation |
| Product Category | Other Modifying Enzymes |
| Reaction Temperature | 37 °C |
| Heat Inactivation | 100 °C (temporarily) |
| Concentration | 0.5 U/μL |
-Alkaline Phosphatase (200 U)
– Reaction buffer (10x)
– Reaction buffer (10x)
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | 5'-dephosphorylation |
| Product Category | Other Modifying Enzymes |
| Reaction Temperature | 37 °C |
| Heat Inactivation | 100 °C (temporarily) |
| Concentration | 0.5 U/μL |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | 5'-dephosphorylation |
| Product Category | Other Modifying Enzymes |
| Reaction Temperature | 37 °C |
| Heat Inactivation | 100 °C (temporarily) |
| Concentration | 0.5 U/μL |
CoA
Certificate of Analysis
