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SKU
MB42001
DNA Polymerase I (E. coli)
DNA Polymerase I (E. coli) is a non-thermostable DNA polymerase with inherent 3´→ 5´ (proofreading) and 5´→ 3´ exonuclease activities, in addition to a lower and non-specific ribonuclease H activity.
Availability
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Dry Ice
DNA Polymerase I (E. coli) is a non-thermostable DNA polymerase with inherent 3´→ 5´ (proofreading) and 5´→ 3´ exonuclease activities, in addition to a lower and non-specific ribonuclease H activity. The 5´→ 3´ exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation. Thus, DNA Polymerase I (E. coli) displays no strand-displacement activity and may be used for DNA labelling by nick-translation, in conjunction with DNase I, or second-strand cDNA synthesis, in conjunction with RNase H. DNA Polymerase I (E. coli) accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labelled nucleotides) as substrates for the DNA synthesis.
Applications:
– DNA labelling by nick-translation
– Second-strand synthesis of cDNA
Applications:
– DNA labelling by nick-translation
– Second-strand synthesis of cDNA
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase, 5' → 3' exonuclease |
Product Category | Polymerases |
Reaction Temperature | 15 - 37 °C |
Heat Inactivation | 75°C |
Concentration | 10 U/μL |
– DNA Polymerase I (E.coli) (10 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase, 5' → 3' exonuclease |
Product Category | Polymerases |
Reaction Temperature | 15 - 37 °C |
Heat Inactivation | 75°C |
Concentration | 10 U/μL |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase, 5' → 3' exonuclease |
Product Category | Polymerases |
Reaction Temperature | 15 - 37 °C |
Heat Inactivation | 75°C |
Concentration | 10 U/μL |
MSDS
Material Safety Data Sheets
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CoA
Certificate of Analysis