T4 DNA Polymerase (MB42201)

Refrigerated package

59,00

150 U

59,00

Description:T4 DNA Polymerase is a typical non-thermostable DNA polymerase displaying a much higher proofreading 3´→5´ exonuclease activity when compared with DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→3´ exonuclease activity. The enzyme is of particular interest for 3´ overhang removal or 5´ overhang fill-in to form blunt ends. T4 DNA Polymerase does not display strand-displacement activity.

Features:
– Higher proofreading 3´→5´ exonuclease activity
– Lacks 5´→3´ exonuclease activity
– No strand displacement activity
– Suitable for generating blunt ends

Applications:
– Removal of 3´ overhang to form blunt ends
– 5´ overhang fill-in to form blunt ends
– Probe labeling

Specifications:

Enzyme concentration:3 U/μL
Source:Recombinant E. coli strain
Optimal reaction temperature:12-37 °C
Heat inactivation:75 °C for 20 min
Storage conditions:Store at -20 °C
Shipping conditions:Shipped at dry ice

Components:
– T4 DNA Polymerase (3 U/μL)
– Reaction Buffer (10x)

Product Brochure EN
Certificate of Analysis ZN011

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