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SKU
MB42301
phi29 DNA Polymerase
phi29 DNA Polymerase is a highly processive non-thermostable polymerase displaying strong strand displacement activity, which allows highly efficient isothermal DNA amplification.
Availability
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Dry Ice
phi29 DNA Polymerase is a highly processive non-thermostable polymerase displaying strong strand displacement activity, which allows highly efficient isothermal DNA amplification. phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilisphage phi29 and possesses a strong 3'→5' exonuclease (proofreading) activity. It acts preferentially on single-stranded DNA or RNA. phi29 DNA Polymerase is particular efficient for Whole Genome Amplification (WGA) through Multiple Displacement Amplification (MDA).
Applications:
– Isothermal amplification
– Whole Genome Amplification (WGA)
– Multiple displacement amplification (MDA)
– DNA template preparation for sequencing
Applications:
– Isothermal amplification
– Whole Genome Amplification (WGA)
– Multiple displacement amplification (MDA)
– DNA template preparation for sequencing
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase, Strand displacement |
Product Category | Polymerases |
Reaction Temperature | 30°C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
– phi29 DNA Polymerase (10 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase, Strand displacement |
Product Category | Polymerases |
Reaction Temperature | 30°C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase, Strand displacement |
Product Category | Polymerases |
Reaction Temperature | 30°C |
Heat Inactivation | 65 °C |
Concentration | 10 U/μL |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis