NZY Tissue gDNA Isolation kit (MB135)

Description: NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample sources including animal cells and tissues, Gram-positive and Gram-negative bacteria, mouse tails, yeast, forensic samples and clinical samples.
The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.

Features:
– High genomic DNA quality
– High yields from multiple sample sources
– Optimized protocols for a range of starting materials
– Fast and easy small-scale protocol
– Silica-membrane technology

Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Multiplex-PCR
– Enzymatic manipulations

Specifications:

Components:
– Buffer NT1
– Buffer NL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– NZYSpin Tissue columns
– Collection tubes