NZY Tissue gDNA Isolation kit (MB135)

159,00529,00

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Description: NZY Tissue gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a variety of sample sources including animal cells and tissues, Gram-positive and Gram-negative bacteria, mouse tails, yeast, forensic samples and clinical samples.
The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses optimized lysis buffers containing Proteinase K and SDS to release DNA from cells. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Tissue Column and others impurities such as proteins and salts are removed during the washing steps. The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.

Features:
– High genomic DNA quality
– High yields from multiple sample sources
– Optimized protocols for a range of starting materials
– Fast and easy small-scale protocol
– Silica-membrane technology

Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Multiplex-PCR
– Enzymatic manipulations

Specifications:

Sample material (general):tissue, bacterial cells, blood
Format:Spin-column
Sample amount:< 25 mg tissue/107 cells
Typical yield:up to 35 µg
A260/A280:1.7-1.9
Elution volume:60-100 µL
Binding capacity:60 µg
Preparation time20 min/preparation (excluding lysis step)
High-throughput compatibility:No
Support protocols:yes (see “Product resources” above)
Product size:50 and 200 columns
Storage conditions:Store all components at room temperature
Shipping conditions:Shipped at room temperature

Components:
– Buffer NT1
– Buffer NL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– NZYSpin Tissue columns
– Collection tubes

Yeast samples protocol
Swabs samples protocol
Stool samples protocol
Product Brochure EN
Paraffin samples protocol
Insect samples protocol
MSDS PT
MSDS EN
Certificate of Analysis ZN081
Certificate of Analysis ZN051
Certificate of Analysis NM091
Certificate of Analysis NM081
Certificate of Analysis NM021

Protocol Overview:

gDNA Tissue_protocol

The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
da Cunha M, Pais SV, Bugalhão JN, Mota LJ
PLoS One, 2017

Does DNA methylation regulate metamorphosis? The case of the sea lamprey (Petromyzon marinus) as an example
Covelo-Soto L, Saura M, Morán P
Comp Biochem Physiol B Biochem Mol Biol, 2015

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