NZYBlood gDNA Isolation kit has been discontinued.
Please check NZYTissue gDNA Isolation kit page as you’ll find a suitable protocol to use with samples such as blood, buffy coat and plasma.
Description: NZY Blood gDNA Isolation kit provides a fast and easy small-scale method for the purification of pure genomic DNA from whole blood, serum, plasma or body fluids. The simple procedure combines the efficiency of an improved lysis buffer (containing Proteinase K) with the selective binding properties of a silica-based column.
This method requires no phenol or chloroform extraction. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Blood Column and other contaminants, such as proteins and salts, are removed during the subsequent washing steps. The highly pure genomic DNA is eluted in an alkaline elution buffer and it is ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.
– High genomic DNA quality
– High yields from whole blood, serum, plasma or body fluids
– Fast and easy small-scale protocol
– Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
– Silica-membrane technology
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– Real-time quantitative PCR (qPCR)
– DNA sequencing
– Enzimatic manipulations
|Sample material :||whole blood (fresh, frozen or treated), serum, plasma, body fluids|
|Sample amount:||up to 200 μL whole blood/5 x 106 leukocyte cells|
|Typical yield||up to 6 μg|
|Elution volume:||60-100 μL|
|Binding capacity:||60 μg|
|Preparation time:||< 30 min|
|Product size:||50 and 200 columns|
|Storage conditions:||Store all components at room temperature|
|Shipping conditions:||Shipped at room temperature|
– Buffer NBL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– NZYSpin Blood columns
– Collection tubes