NZY Blood gDNA Isolation kit (MB136)

153,00529,00

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NZYBlood gDNA Isolation kit has been discontinued.
Please check NZYTissue gDNA Isolation kit page as you’ll find a suitable protocol to use with samples such as blood, buffy coat and plasma.

 

Description: NZY Blood gDNA Isolation kit provides a fast and easy small-scale method for the purification of pure genomic DNA from whole blood, serum, plasma or body fluids. The simple procedure combines the efficiency of an improved lysis buffer (containing Proteinase K) with the selective binding properties of a silica-based column.
This method requires no phenol or chloroform extraction. After preparing the lysate, DNA is selectively absorbed into the NZYSpin Blood Column and other contaminants, such as proteins and salts, are removed during the subsequent washing steps. The highly pure genomic DNA is eluted in an alkaline elution buffer and it is ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.

Features:
– High genomic DNA quality
– High yields from whole blood, serum, plasma or body fluids
– Fast and easy small-scale protocol
– Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
– Silica-membrane technology

Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Multiplex-PCR
– Enzimatic manipulations

Specifications:

Sample material :whole blood (fresh, frozen or treated), serum, plasma, body fluids
Format:Spin-column
Sample amount:up to 200 μL whole blood/5 x 106 leukocyte cells
Typical yieldup to 6 μg
A260/A280:1.7-1.9
Elution volume:60-100 μL
Binding capacity:60 μg
Preparation time:< 30 min
High-throughput compatibility:No
Product size:50 and 200 columns
Storage conditions:Store all components at room temperature
Shipping conditions:Shipped at room temperature

Components: 
– Buffer NBL
– Buffer NW1
– Buffer NW2 (concentrated)
– Buffer NE
– Proteinase K (lyophilized)
– Proteinase buffer
– NZYSpin Blood columns
– Collection tubes

Product Brochure EN
MSDS PT
MSDS EN
Certificate of Analysis NM081
Certificate of Analysis MB13604 NM021
Certificate of Analysis MB13603 NM021
Certificate of Analysis MB13602 NM021

Protocol Overview:

gDNA Blood_protocol

Dosage of Single Low-Dose Primaquine to Stop Malaria Transmission
Cruz M, Sánchez IM, Diaz J, Cuevas F, Silva M, Disla M, Ferreira PE, Veiga MI.
J Infect Dis, 2018

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