NZY Soil gDNA Isolation kit (MB21802)

Description: NZY Soil gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of soil microorganisms, such as bacteria, archaea, fungi, and algae in soil, sludge and sediment samples. This kit is suitable for samples from forest, bog, farmland, grassland and stool samples. The samples are mechanically disrupted using ceramic beads. Proteins and other PCR inhibitors in solution are precipitated with Buffer NSL3 followed by centrifugation with the ceramic beads. Any residual humic substances and other PCR inhibitors are efficiently removed in the washing steps. High yields with excellent purity from all types of samples are possible due to the combination of Buffer NSL1 and Buffer NSL2 with the additive NS enhancer. NZY Soil gDNA Isolation kit is optimized to isolate 2-10 µg of DNA from up to 500 mg of soil or sediment.

Features:
– High DNA yield and quality obtained from a range of samples, by combining two alternative lysis buffers with a enhancer solution
– Efficient sample lysis provided by ceramic beads (included in NZYSpin Soil Bead Tubes)
– Silica-membrane technology
– Efficient removal of PCR inhibitors using NZYSpin Soil Inhibitor Removal Columns

Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Southern blotting
– Microarray technology
– Enzimatic manipulations

Specifications:

Components:
– Buffer NSL1
– Buffer NSL2
– Buffer NSL3
– NS Enhancer
– Buffer NSB
– Buffer NSW1
– Buffer NSW2 (concentrate)
– Buffer NSE
– NZYSpin Soil Bead Tubes
– NZYSpin Soil Inhibitor Removal Columns
– NZYSpin Soil Columns
– Collection Tubes (2 mL)
– Collection tubes (2 mL, lid)