NZY Soil gDNA Isolation kit (MB21802)

359,00

50 columns

359,00

Description: NZY Soil gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of soil microorganisms, such as bacteria, archaea, fungi, and algae in soil, sludge and sediment samples. This kit is suitable for samples from forest, bog, farmland, grassland and stool samples. The samples are mechanically disrupted using ceramic beads. Proteins and other PCR inhibitors in solution are precipitated with Buffer NSL3 followed by centrifugation with the ceramic beads. Any residual humic substances and other PCR inhibitors are efficiently removed in the washing steps. High yields with excellent purity from all types of samples are possible due to the combination of Buffer NSL1 and Buffer NSL2 with the additive NS enhancer. NZY Soil gDNA Isolation kit is optimized to isolate 2-10 µg of DNA from up to 500 mg of soil or sediment.

Features:
– High DNA yield and quality obtained from a range of samples, by combining two alternative lysis buffers with a enhancer solution
– Efficient sample lysis provided by ceramic beads (included in NZYSpin Soil Bead Tubes)
– Silica-membrane technology
– Efficient removal of PCR inhibitors using NZYSpin Soil Inhibitor Removal Columns

Applications:
The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– PCR
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Southern blotting
– Microarray technology
– Enzimatic manipulations

Specifications:

Sample material (general): soil, sludge and sediment
Format: Spin-column
Sample amount: < 500 mg soil
Typical yield: 2-10 µg
A260/A280: 1.7-1.9
Elution volume: 30-100 µL
Binding capacity: 50 µg
Preparation time approx. 15 min/preparation (excluding lysis step)
High-throughput compatibility: No
Product size: 50 columns
Storage conditions: Store all components at room temperature
Shipping conditions: Shipped at room temperature

Components:
– Buffer NSL1
– Buffer NSL2
– Buffer NSL3
– NS Enhancer
– Buffer NSB
– Buffer NSW1
– Buffer NSW2 (concentrate)
– Buffer NSE
– NZYSpin Soil Bead Tubes
– NZYSpin Soil Inhibitor Removal Columns
– NZYSpin Soil Columns
– Collection Tubes (2 mL)
– Collection tubes (2 mL, lid)

Product Brochure EN
MSDS PT
MSDS EN
Certificate of Analysis ZN084
Certificate of Analysis ZN083
Certificate of Analysis ZN082
Certificate of Analysis ZN081
Certificate of Analysis YO042
Certificate of Analysis YO041
Certificate of Analysis NM082
Certificate of Analysis NM081

Protocol overview:

Soil_gDNA_protocol_overview

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