Description: NZYMiniprep kits are designed for the rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. NZYTech´s miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYTech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. NZYMiniprep kit includes an additional washing buffer (AY) which is strongly recommended for the complete removal of high levels of endonucleases. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 10 mL of E. coli cultures and double the volumes of Buffers A1, A2 and A3.
– Quick mini-prep of pure plasmid DNA
– Spin column silica-based
– High yields of plasmid DNA (15-45 µg of high-copy number plasmid DNA/ column)
– Prevents the use of toxic substances (e.g. phenol, chloroform or CsCl)
– Contains an endonuclease-removal buffer (AY washing buffer)
The purified plasmid DNA is suitable for use in the most demanding molecular biology applications, including:
– Automated fluorescent or manual sequencing
– In vitro transcription
– Enzymatic reactions
High-purity plasmid DNA mini preparation:
Isolation of low-copy and high-copy number plasmid DNA (in duplicate) using NZYMiniprep or a competitor plasmid purification kit. Lane M: NZYDNA Ladder III.
|Culture volume/starting material:||1-10 mL E. coli culture volume|
|Plasmid type:||high- and low-copy number, BACs or cosmids|
|Yield:||up to 45 µg|
|Elution volume:||30-50 µL|
|Binding capacity:||50 µg|
|Procedure:||Manual – centrifugation|
|Preparation time:||< 25 min|
|Product size:||50, 200, 2×50 and 5×200 columns|
|High-throughput compatibility:||NZYMiniprep 96 well plate|
|Storage conditions:||All kit components can be stored at room temperature; Buffer A1 with RNase should be stored at 4 °C for frequent use|
|Shipping conditions:||Shipped at room temperature|
(*) For most convenience, NZYTech also sells all the components of the kit (except Buffer AE) in separate.