We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Only %1 left
SKU
NZYSpeedy Miniprep
NZYSpeedy Miniprep kit is designed for the ultra-rapid preparation of highly pure plasmid DNA from E. coli strains.
Availability
Ready to Ship
NZYSpeedy Miniprep kit is designed for the ultra-rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia colistrains. NZYtech´s speedy miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto a new specially treated silica membrane in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYtech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. The eluted DNA is suitable for applications like automated fluorescent sequencing, PCR and a wide range of other enzymatic manipulations. NZYSpeedy Miniprep kit includes a newly developed washing buffer (AS) which ensures complete removal of contaminants in a rapid washing step.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 15 min |
– Buffer A1
– Buffer A2
– Buffer A3
– Buffer AS (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
– Buffer A2
– Buffer A3
– Buffer AS (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 15 min |
Plasmid DNA was isolated from 3 mL of a recombinant E. coli strain using three different rapid protocols (NZYTech, lanes 1-4; Competitor 1, lanes 5-8; Competitor 2, lanes 9-12). DNA isolation was performed according to each manufacturer ́s protocol. The quadruplicates (2 μL of 50 μL eluate) were analysed through agarose gel electrophoresis (A). Lane M: NZYDNA Ladder III. Eluates were also analysed by UV/VIS spectroscopy to determine total yields (B).
Protocol Overview:
Protocol Overview:
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 15 min |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 15 °C to 25 °C |
| Application | In vitro transcription, Cloning, PCR, Sequencing, Transformation |
| Starting Material | E. coli culture |
| Scale | Miniprep |
| Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number |
| Format | Column |
| Product Category | DNA Purification, Plasmid DNA Purification |
| Isolation Technology | Silica membrane |
| Protocol time | Approx. 15 min |
CoA
Certificate of Analysis
