NZY Total RNA Isolation kit (MB13402)

199,00

50 columns

199,00

Get Any 4, Pay 3 - October 2021
We offer the cheapest product. Not valid for services.

Description: NZY Total RNA Isolation kit is designed for the easy purification of total RNA of highest integrity (longer than 200 bases) from a variety of sources such as animal tissues, bacteria cells and cell cultures. This method uses a denaturing lysis buffer containing guanidine thiocyanate, which inactivates cellular RNases, to ensure the recovery of intact RNA molecules. Ethanol is added to provide selective binding of total RNA into the silica membrane column and impurities are efficiently washed away. To prevent the contamination with DNA, a DNase I solution is directly added onto the silica membrane of the binding column. High-quality RNA is then eluted in RNase-free water.

Features:
– High RNA quality
– Ready-to-use Total RNA
– High yields from multiple sample sources (animal tissues, yeast, bacterial cells and cell cultures)
– Optimized protocols for a range of starting materials
– High-speed protocol
– Silica-membrane technology
– DNase I included

Applications:
The purified RNA is suitable for use in the most demanding molecular biology applications, including:
– Reverse transcriptase PCR (RT-PCR)
– Real-time quantitative PCR (qPCR)
– in vitro translation
– cDNA synthesis

Specifications:

Sample material (general):tissue, yeast, bacterial cells, cell cultures
Format:Spin-column
Sample amount:up to 30 mg tissue/1 x 109 bacterial cells/5 x 106 cultured cells/108 yeast cells
Typical yield:up to 70 μg
A260/A280:1.9-2.1
Elution volume:40-60 μL
Binding capacity:200 μg
Preparation time:35 min
High-throughput compatibility:No
Support protocols:yes (see “Product resources” above)
Product size:50 columns
Storage conditions:Store all components at room temperature
Shipping conditions:Shipped at room temperature

Components:
– Buffer NR
– Buffer NI
– Buffer NRW1
– Buffer NRW2 (concentrated)
– RNase free water
– Digestion buffer
– DNase I (lyophilized)
– NZYSpin Homogenization Columns (purple rings)
– NZYSpin Binding Columns (blue rings)
– Collection tubes (1.5 mL and 2 mL)

Yeast samples protocol
Product Brochure EN
Paraffin samples protocol
Biological fluids protocol
MSDS PT
MSDS EN
Certificate of Analysis ZN081
Certificate of Analysis ZN051
Certificate of Analysis YL121
Certificate of Analysis NM091
Certificate of Analysis NM071
Certificate of Analysis NM031

Protocol Overview for purification of total RNA from animal tissues or cells:

RNA_purification_protocol_overviw

β-Cryptoxanthin Inhibits Angiogenesis in Human Umbilical Vein Endothelial Cells Through Retinoic Acid Receptor.
Quesada-Gómez JM, Santiago-Mora R, Durán-Prado M, Dorado G, Pereira-Caro G, Moreno-Rojas JM, Casado-Díaz A
Mol Nutr Food Res, 2018

Estrogen Modulates Glycerol Permeability in Sertoli Cells through Downregulation of Aquaporin-9
Bernardino RL, Carrageta DF, Silva AM, Calamita G, Alves MG, Soveral G, Oliveira PF
Cells, 2018

The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
da Cunha M, Pais SV, Bugalhão JN, Mota LJ
PLoS One, 2017

The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells
Cipriano M, Correia JC, Camões SP, Oliveira NG, Cruz P, Cruz H, Castro M, Ruas JL, Santos JM, Miranda JP
Arch Toxicol, 2017

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