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SKU
MB18501
NZYol
NZYol is a ready-to-use reagent combining a blend of phenol and other components for the isolation of total RNA from cells and tissues.
Availability
Ready to Ship
NZYol is a ready-to-use reagent combining a blend of phenol and other components for the isolation of total RNA from cells and tissues. NZYol disrupts cells exposing cellular components while maintaining the integrity of the extracted RNA. Biological samples are homogenized or lysed in NZYol and then separated into organic and aqueous phases. RNA remains in the aqueous phase and is subsequently recovered by precipitation with isopropyl alcohol. Isolated RNA is suitable for any downstream application such as RT-PCR, hybridization assays, or in vitro translation. 1 mL of NZYol is sufficient to isolate Total RNA from 1 x 107 cells or 100 mg of tissue. The expected yield of RNA from 1 mg of tissue is: 2-5 μg from mouse kidney; 5-10 μg from mouse liver. The expected yield of RNA from 1 x 106; cultured cells is: 8-15 μg from epithelial cells; 20-25 μg from cell fibroblasts.
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 2 °C to 8 °C |
| Application | In vitro translation, cDNA synthesis, PCR, qPCR, RT-PCR, RT-qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Plant tissue |
| Features | Manual, Ready-to-use |
| Format | Solution |
| Product Category | Protein Purification, RNA Purification |
| Isolation Technology | Organic Extraction |
Molecular Characterization of Portuguese Patients with Hereditary Cerebellar Ataxia
Santos M, Damásio J, Carmona S, Neto JL, Dehghani N, Guedes LC, Barbot C, Barros J, Brás J, Sequeiros J, Guerreiro R.
Cells, 2022
Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype
Santos M, Damásio J, Kun-Rodrigues C, Barbot C, Sequeiros J, Brás J, Alonso I, Guerreiro R.
J Clin Med , 2020
Progressive Microcephaly, Spasticity and Development Delay: Novel SLC1A4 Variants in Two Portuguese Families and Literature Review
Teixeira J, Dória S, Santos M, Alonso I, Leão M.
J Genet Genom Sci , 2020
Tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern Portugal
Pereira A, Parreira R, Cotão AJ, Nunes M, Vieira ML, Azevedo F, Campino L, Maia C.
Ticks Tick Borne Dis, 2018
Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures
Santos RB, Pires AS, Abranches R.
Sci Rep, 2017
Santos M, Damásio J, Carmona S, Neto JL, Dehghani N, Guedes LC, Barbot C, Barros J, Brás J, Sequeiros J, Guerreiro R.
Cells, 2022
Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype
Santos M, Damásio J, Kun-Rodrigues C, Barbot C, Sequeiros J, Brás J, Alonso I, Guerreiro R.
J Clin Med , 2020
Progressive Microcephaly, Spasticity and Development Delay: Novel SLC1A4 Variants in Two Portuguese Families and Literature Review
Teixeira J, Dória S, Santos M, Alonso I, Leão M.
J Genet Genom Sci , 2020
Tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern Portugal
Pereira A, Parreira R, Cotão AJ, Nunes M, Vieira ML, Azevedo F, Campino L, Maia C.
Ticks Tick Borne Dis, 2018
Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures
Santos RB, Pires AS, Abranches R.
Sci Rep, 2017
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 2 °C to 8 °C |
| Application | In vitro translation, cDNA synthesis, PCR, qPCR, RT-PCR, RT-qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Plant tissue |
| Features | Manual, Ready-to-use |
| Format | Solution |
| Product Category | Protein Purification, RNA Purification |
| Isolation Technology | Organic Extraction |
| Shipping Conditions | Room Temperature |
|---|---|
| Storage Conditions | 2 °C to 8 °C |
| Application | In vitro translation, cDNA synthesis, PCR, qPCR, RT-PCR, RT-qPCR, Sequencing |
| Starting Material | Animal cells, Animal Tissue, Plant tissue |
| Features | Manual, Ready-to-use |
| Format | Solution |
| Product Category | Protein Purification, RNA Purification |
| Isolation Technology | Organic Extraction |
CoA
Certificate of Analysis
