NZYol (MB18501)


100 mL


Description: NZYol is a ready-to-use reagent combining a blend of phenol and other components for the isolation of total RNA from cells and tissues. NZYol disrupts cells exposing cellular components while maintaining the integrity of the extracted RNA. Biological samples are homogenized or lysed in NZYol and then separated into organic and aqueous phases. RNA remains in the aqueous phase and is subsequently recovered by precipitation with isopropyl alcohol. Isolated RNA is suitable for any downstream application such as RT-PCR, hybridization assays, or in vitro translation. 1 mL of NZYol is sufficient to isolate Total RNA from 1 x 107 cells or 100 mg of tissue. The expected yield of RNA from 1 mg of tissue is: 2-5 μg from mouse kidney; 5-10 μg from mouse liver. The expected yield of RNA from 1 x 106; cultured cells is: 8-15 μg from epithelial cells; 20-25 μg from cell fibroblasts.

– Quick isolation of high quality RNA
– Ready-to-use solution ideal for a wide variety of cells and tissues, including animal and plant tissues rich in polysaccharides and peptidoglycans
– Cost-effective
– 1 hour protocol
– Convenient and reliable
– Pure RNA is ideal for any downstream applications including RT-PCR and in vitro translation

– Isolation of total RNA

– Storage conditions: Store at 4 ºC (or at room temperature for short periods of time)
– Shipping conditions: Shipped at room temperature

Product Brochure EN
Certificate of Analysis ZN021
Certificate of Analysis YL121

Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype
Santos M, Damásio J, Kun-Rodrigues C, Barbot C, Sequeiros J, Brás J, Alonso I, Guerreiro R
J Clin Med, 2020

Progressive Microcephaly, Spasticity and Development Delay: Novel SLC1A4 Variants in Two Portuguese Families and Literature Review
Teixeira J, Dória S, Santos M, Alonso I, Leão M
J Genet Genomic Sci, 2020

Tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern Portugal
Pereira A, Parreira R, Cotão AJ, Nunes M, Vieira ML, Azevedo F, Campino L, Maia C
Ticks and Tick-borne Diseases, 2018

Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures
Santos RB, Pires AS, Abranches R
Scientific Reports, 2017

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