NZYMidiprep (MB050)




Description: NZYMidiprep kit is designed for the rapid, medium-scale preparation of highly pure plasmid DNA (typically 100 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYTech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 100 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.

– Quick midi-prep of pure plasmid DNA
– Column silica-based
– High yields of plasmid DNA (80-150 µg of high-copy number plasmid DNA/ column)

The purified plasmid DNA is suitable for use in the most demanding molecular biology applications, including:
– Transfection
– In vitro transcription
– Enzymatic reactions
– Automated fluorescent or manual sequencing
– Cloning
– Hybridization

High-purity plasmid DNA midi preparation:

midiprep2Isolation of high-copy number plasmid DNA (in duplicate) using NZYMidiprep (1) or a competitor plasmid purification kit (2). Lane M: NZYDNA Ladder III.



Culture volume/starting material:25-100 mL E. coli culture volume
Plasmid type:high- and low-copy number, BACs or cosmids
Format:gravity flow column
Yield:up to 150 µg
Elution volume:variable
Procedure:Manual – centrifugation
Preparation time:approx. 70 min
Product size:5, 20 and 3×20 columns
Storage conditions:All kit components can be stored at room temperature; Buffer M1 with RNase should be stored at 4 °C for frequent use
Shipping conditions:Shipped at room temperature

Components (*):
– Buffer M1
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Midi Columns

(*) For most convenience, NZYTech also sells in separate the buffers (NZYMidiprep, no columns) and NZYTech Plasmid Midi Columns

Product Brochure EN
Certificate of Analysis YL123
Certificate of Analysis YL122
Certificate of Analysis YL121
Certificate of Analysis NM081

The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion
da Cunha M, Pais SV, Bugalhão JN, Mota LJ
PLoS One, 2017

Structure-based virtual screening toward the discovery of novel inhibitors of the DNA repair activity of the human apurinic/apyrimidinic endonuclease 1
Guerreiro PS, Estácio SG, Antunes F, Fernandes AS, Pinheiro PF, Costa JG, Castro M, Miranda JP, Guedes RC, Oliveira NG.
Chem Biol Drug Des, 2016

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