– Fast amplification (15 sec/kb)
– Proofreading activity
– High-fidelity PCR
– Fast PCR
– Generation of products for blunt-end cloning
Successful amplification with limited extension time and proofreading activity:
Three different DNA fragments of various sizes and from different origins were amplified using a limited extension period of 15 sec/kb and 2.5 U of NZYSpeedy Proof DNA polymerase.
Amplification of nucleic acids from different DNA templates using NZYSpeedy Proof DNA polymerase. Lane 1: 350 bp PCR product (sheep genomic DNA, 120 ng). Lane 2: 1 kb PCR product (E. coli genomic DNA, 100 ng). Lane 3: 2 kb PCR product (plasmid DNA, 10 ng). Lane M: NZYDNA Ladder III.
|Product length:||0-3 kb|
|Hot-start like capacity:||no|
|Storage conditions:||Store at -20 ºC|
|Shipping conditions:||Shipped at 4 ºC to dry ice|
– NZYSpeedy Proof DNA polymerase (2.5 U/μL)
– Reaction buffer (10x)
– MgCl2 (50 mM)