Supreme NZYProof 2x Green Master Mix (MB285)


Description: Supreme NZYProof 2× Green Master Mix is a premixed ready-to-use solution containing Supreme NZYProof DNA polymerase (MB283), an engineered highly accurate, fast and sensitive variant of NZYProof DNA polymerase displaying a hot-start like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as the degradation of primers and template DNA during PCR reaction setup. Thus, Supreme NZYProof DNA polymerase generates higher specificity, sensitivity and yield during the accurate amplification of DNA. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of DNA templates by PCR. Mg2+ final concentration is 1.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with Supreme NZYProof 2× Green Master Mix may be directly loaded onto agarose gels. Supreme NZYProof 2× Green Master Mix contains two dyes (blue and yellow) that allow monitoring the progress of electrophoresis.

Supreme NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading activity. Resulting PCR products have blunt-ends and are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).

– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Hot-start like capacity
– Proofreading activity

– High-fidelity PCR
– Hot-start PCR
– Routine PCR
– Generation of products for blunt-end cloning

Increased sensitivity of Supreme NZYProof 2x Green Master Mix
Superior sensitivity of Supreme NZYProof DNA polymerase present in the 2x Green Master Mix allows amplification using few amounts of DNA template.


A 1 kb DNA fragment was amplified using 2-fold Escherichia coli genomic DNA dilutions ranging from 40 ng to 39 pg (lanes 1-11) with Supreme NZYProof 2x Green Master Mix and a similar competitor. NC: No template control. Lane M: NZYDNA Ladder III (MB044).



Product length: 0-10 kb
Hot-start like capacity: yes
Extension time: 15-30 sec/kb at 72 °C
3´→5´ activity: yes
Product overhang: blunt
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice

Supreme NZYProof 2× Green Master Mix (0.2 U/μL)

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis YO091
Certificate of Analysis YO021
Certificate of Analysis YL101

1. What to do when there is no product amplification or low yield?
This can be due to the following situations:

a) Inadequate annealing temperature. The reaction mix composition may affect the melting properties of primers and DNA. Adjust the annealing temperature to accommodate the primer with the lowest melting temperature (5 ° to 10 °C lower than Tm).
b) Presence of PCR inhibitors. Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration.

2. How should I store my Supreme NZYProof 2x Green Master Mix?
The master mix should be stored at -20 ºC, in a constant temperature freezer. Minimize the number of freeze-thaw cycles by storing in working aliquots. The Mix maybe stored at 4 °C for up to 7 days.

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