NZYProof 2x Green Master Mix (MB287)

100,00850,00

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Description: NZYProof 2× Green Master Mix is a premixed ready-to-use solution containing NZYProof DNA polymerase, an enzyme that presents high fidelity and displays great performance in the majority of PCR applications. NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading capacity which enables the polymerase to amplify DNA with increased accuracy. The enzyme is highly efficient in the amplification of longer (≤10 kb) PCR products and site-directed mutagenesis. In addition, it is the recommended polymerase for routine cloning that requires precision. The master mix was optimized to allow high yields and enhanced sensitivity due the presence of stabilizer solution. It contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of DNA templates by PCR. Mg2+ final concentration is 1.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with NZYProof 2× Green Master Mix may be directly loaded onto agarose gels. NZYProof 2× Green Master Mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis.

NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading activity. Resulting PCR products have blunt-ends and are suitable for cloning with NZYTech´s NZY-blunt PCR cloning kit (MB121).

Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Proofreading activity

Applications:
– High-fidelity PCR
– Routine PCR
– Generation of products for blunt-end cloning

 Specifications:

Product length:0-10 kb
Hot-start like capacity:no
Extension time:60 sec/kb at 72 °C
3´→5´ activity:yes
Product overhang:blunt
Storage conditions:Store at -20 ºC
Shipping conditions:Shipped at 4 ºC to dry ice

Components:
NZYProof 2× Green Master Mix (0.2 U/μL)

Product Brochure EN
Safety Information EN
MSDS PT
MSDS EN
Informacao de Seguranca PT
Certificate of Analysis YL101

1. What to do when there is no product amplification or low yield?
This can be due to the following situations:

a) Inadequate annealing temperature. The reaction mix composition may affect the melting properties of primers and DNA. Adjust the annealing temperature to accommodate the primer with the lowest melting temperature (5 ° to 10 °C lower than Tm).
b) Presence of PCR inhibitors. Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration.

2. How should I store my NZYProof 2x Green Master Mix?
The master mix should be stored at -20 ºC, in a constant temperature freezer. Minimize the number of freeze-thaw cycles by storing in working aliquots. The Mix maybe stored at 4 °C for up to 7 days.

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