NZYTaq II 2x Colourless Master Mix

Storage Conditions:

-85°C to -15°C

Availability

Ready to Ship

Details

NZYTaq II 2× Colourless Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. For highly sensitive downstream applications it is recommended to purify the amplified PCR product using NZYGelpure (MB011) before usage in subsequent protocols. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Extension time: 15-30 sec/kb at 72 °C
3'→5' activity: no
Product overhang: 3'-A

More Information
Shipping Conditions	Room Temperature
Storage Conditions	-85°C to -15°C
Application	Colony PCR, Routine PCR, TA Cloning
Features	Standard
Product Length	0-6 kb
Format	Mastermix
Product Category	End-Point PCR
Concentration	0.2 U/μL
Hot Start	No

Components

NZYTaq II 2× Colourless Master Mix (0.2 U/μL)

More Information
Shipping Conditions	Room Temperature
Storage Conditions	-85°C to -15°C
Application	Colony PCR, Routine PCR, TA Cloning
Features	Standard
Product Length	0-6 kb
Format	Mastermix
Product Category	End-Point PCR
Concentration	0.2 U/μL
Hot Start	No

Citations

A Potential Atypical Case of Rabbit Haemorrhagic Disease in a Dwarf Rabbit
Abade dos Santos FA, Magro C, Carvalho CL, Ruivo P, Duarte MD, Peleteiro M
Animals, 2020

More Information
Shipping Conditions	Room Temperature
Storage Conditions	-85°C to -15°C
Application	Colony PCR, Routine PCR, TA Cloning
Features	Standard
Product Length	0-6 kb
Format	Mastermix
Product Category	End-Point PCR
Concentration	0.2 U/μL
Hot Start	No

FAQs

NZYTaq II 2x Colourless Master Mix

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NZYTaq II 2x Colourless Master Mix

What to do when there is no product amplification or low yield?

This can be due to the following situations:
a) Inadequate annealing temperature. The reaction mix composition may affect the melting properties of primers and DNA. Adjust the annealing temperature to accommodate the primer with the lowest melting temperature (5 °C to 10 °C lower than Tm).
b) Presence of PCR inhibitors. Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration.
c) Concentration of magnesium is too low. Mg2+ is included in the Master Mix at a final concentration of 2.5 mM, which is sufficient for most targets. For some targets, higher Mg2+ concentration may be required. Titrate from 2.5 mM to 4 mM (final concentration) in 0.5 mM increments. (Note: MgCl2 is not provided in separate tubes).

How can I reduce the number of non-specific bands?

Adjust annealing conditions and/or design another set of primers, by increasing the length and avoiding complementary sequences.

How should I store my NZYTaq II 2× Colourless Master Mix?

The product should be stored at -20 ºC, in a constant temperature freezer.

More Information
Shipping Conditions	Room Temperature
Storage Conditions	-85°C to -15°C
Application	Colony PCR, Routine PCR, TA Cloning
Features	Standard
Product Length	0-6 kb
Format	Mastermix
Product Category	End-Point PCR
Concentration	0.2 U/μL
Hot Start	No

More Information
Shipping Conditions	Room Temperature
Storage Conditions	-85°C to -15°C
Application	Colony PCR, Routine PCR, TA Cloning
Features	Standard
Product Length	0-6 kb
Format	Mastermix
Product Category	End-Point PCR
Concentration	0.2 U/μL
Hot Start	No

MSDS

Material Safety Data Sheets

MSDS EN MSDS PT

CoA

Certificate of Analysis

Manuals

Instructions for Use - EN

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