NZYTaq II 2x Green Master Mix (MB358)

40,00339,00

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Description: NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. In addition, reactions assembled with NZYTaq II 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis.

Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– High yields with minimal optimization
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang

Applications:
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning

Specifications:

Product length:0-6 kb
Hot-start-like capacity:no
Extension time:15-30 sec/kb at 72 °C
3´→5´ activity:no
Product overhang:3’-A
Storage conditions:Store at -20 ºC
Shipping conditions:Shipped at room temperature to dry ice

Components:
NZYTaq II 2× Green Master Mix (0.2 U/μL)

Product Brochure EN
Safety Information EN
MSDS PT
MSDS EN
Informacao de Seguranca PT
Certificate of Analysis ZN051
Certificate of Analysis YL101
Certificate of Analysis NM111
Certificate of Analysis NM061

1. What to do when there is no product amplification or low yield?
This can be due to the following situations:
a) Inadequate annealing temperature. The reaction mix composition may affect the melting properties of primers and DNA. Adjust the annealing temperature to accommodate the primer with the lowest melting temperature (5 °C to 10 °C lower than Tm).
b) Presence of PCR inhibitors. Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration.
c) Concentration of magnesium is too low. Mg2+ is included in the Master Mix at a final concentration of 2.5 mM, which is sufficient for most targets. For some targets, higher Mg2+ concentration may be required. Titrate from 2.5 mM to 4 mM (final concentration) in 0.5 mM increments. (Note: MgCl2 is not provided in separate tubes).

2. How can I reduce the number of non-specific bands?
Adjust annealing conditions and/or design another set of primers, by increasing the length and avoiding complementary sequences.

3. How should I store my NZYTaq II 2× Green Master Mix?
The product should be stored at -20 ºC, in a constant temperature freezer.

Preliminary comparative analysis of the resolving power of COX1 and
16S-rDNA as molecular markers for the identification of ticks from Portugal

Filipe D, Parreira R, Pereira A, Galvão N, Cristovão MJ, Nunes M, Vieira LM, Campino L, Maia, C
Veterinary Parasitology: Regional Studies and Repors, 2021

Structural diversity of photoautotrophic populations within the UNESCO site ‘Old Cathedral of Coimbra’ (Portugal), using a combined approach
Soares F, Portugal A, Trovão J, Coelho C, Mesquita N, Pinheiro AC, Gil F, Catarino L, Cardoso SM, Tiago I
International Biodeterioration & Biodegradation, 2019

Description of Aeminiaceae fam. nov., Aeminium gen. nov. and Aeminiumludgeri sp. nov. (Capnodiales), isolated from a biodeteriorated art-piece in the Old Cathedral of Coimbra, Portugal
Trovão J, Tiago I, Soares F, Paiva DS, Mesquita N, Coelho C, Catarino L, Gil F, Portugal A
MycoKeys, 2019

Fungal diversity and distribution across distinct biodeterioration phenomena in limestone walls of the old cathedral of Coimbra, UNESCO World Heritage Site
Trovão J, Portugal A, Soares F, Paiva DS, Mesquita N, Coelho C, Pinheiro AC, Catarino L, Gil F, Tiago I
International Biodeterioration & Biodegradation, 2019

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