NZYTaq II 2x Green Master Mix (MB358)


Description: NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. In addition, reactions assembled with NZYTaq II 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis.

Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– High yields with minimal optimization
– Robust amplification using a wide range of DNA templates
– Leaves an A-overhang

– Powerful PCR
– Colony PCR
– Generation of products for TA cloning


Product length: 0-6 kb
Hot-start-like capacity: no
Extension time: 15-30 sec/kb at 72 °C
3´→5´ activity: no
Product overhang: 3’-A
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at room temperature to dry ice

NZYTaq II 2× Green Master Mix (0.2 U/μL)

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN101
Certificate of Analysis ZN051
Certificate of Analysis YO093
Certificate of Analysis YO092
Certificate of Analysis YO091
Certificate of Analysis YO072
Certificate of Analysis YO071
Certificate of Analysis YO061
Certificate of Analysis YO033
Certificate of Analysis YO032
Certificate of Analysis YL101
Certificate of Analysis NM111
Certificate of Analysis NM061

1. What to do when there is no product amplification or low yield?
This can be due to the following situations:
a) Inadequate annealing temperature. The reaction mix composition may affect the melting properties of primers and DNA. Adjust the annealing temperature to accommodate the primer with the lowest melting temperature (5 °C to 10 °C lower than Tm).
b) Presence of PCR inhibitors. Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration.
c) Concentration of magnesium is too low. Mg2+ is included in the Master Mix at a final concentration of 2.5 mM, which is sufficient for most targets. For some targets, higher Mg2+ concentration may be required. Titrate from 2.5 mM to 4 mM (final concentration) in 0.5 mM increments. (Note: MgCl2 is not provided in separate tubes).

2. How can I reduce the number of non-specific bands?
Adjust annealing conditions and/or design another set of primers, by increasing the length and avoiding complementary sequences.

3. How should I store my NZYTaq II 2× Green Master Mix?
The product should be stored at -20 ºC, in a constant temperature freezer.

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