Description: The Low Molecular Weight Protein Marker II for SDS electrophoresis is a liquid mixture of eight purified proteins for use in molecular weight estimation in the presence of the detergent sodium dodecyl sulphate (SDS). This protein marker contains no oligosaccharides that may cause anomalous migrations and heterogeneous fuzzy bands. The molecular mass of the protein under investigation is determined by comparing its electrophoretic mobility with that of proteins contained in the kit. Three (MB21401) or six (MB21402) 500 µL vials are supplied, each containing a mixture of highly purified protein standards of molecular mass ranging from 11000 to 96000 when used in denaturing polyacrylamide electrophoresis. The mixture is supplied in a loading buffer for direct loading on gels.
Features:
– Ready-to-load form
– Precisely defined size intervals between bands
Applications:
– Estimation of protein molecular weight by SDS-PAGE
Specifications:
– Size Range: 11 – 96 kDa
– Stain type: Unstained
– Storage conditions: Store at -20 °C
– Shipping conditions: Shipped at 4 ºC
Components:
– 3 x 500 µL (300 applications of 5 µL each) or 6 x 500 µL (600 applications of 5 µL each) provided in loading buffer consisting of 30% (w/v) glycerol, 2% (w/v) SDS, 25 mM NaHepes, pH 7.5, 50 mM DTT, 5 mM EDTA, 0.02% (w/v) NaN3
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Sodium Chloride (NaCl)
Tryptone
Yeast Extract micro granulated
SOB Broth
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