GreenSafe Premium (MB13201)

79,00

1 mL

79,00

Description: GreenSafe Premium is a new nucleic acid stain that can be used as a safer alternative to the traditional ethidium bromide for detecting nucleic acids in agarose gels. It is as sensitive as ethidium bromide and can be used exactly in the same way in agarose gel electrophoresis. GreenSafe Premium stain emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (≈270 nm and ≈290 nm) and one strong excitation peak at 490 nm. The fluorescence emission is similar to ethidium bromide when bound to DNA at 530 nm. Thus GreenSafe Premium stain is compatible with a wide variety of gel reading instruments.

Features:
– Used for detecting dsDNA and RNA
– Non-toxic, non-mutagenic and non-carcinogenic (it is an alternative to the ethidium bromide staining)
– High sensitivity even for small fragments
– Superior signal to noise ratio
– Convenient ready-to-use
– Compatible with a wide variety of gel reading instruments
– No hazardous waste

Applications:
– Detection of DNA or RNA in agarose gels by pre-staining or post-staining

High sensitivity over a wide range of DNA sizes:

GreenSafe Premium_figure1

1% (w/v) agarose gel stained with GreenSafe Premium

Specifications:

Target molecule: DNA and RNA
Detection method: Fluorescence
Mode of use: Add stain during gel casting or alternatively gels can be post-stained
Disposal: Waste must be disposed in accordance with environmental control regulations
Storage conditions: Store at room temperature or 4°C, protected from light
Shipping conditions: Shipped at room temperature to 4 ºC

Components:
– 1 vial of 1 mL

MSDS EN
MSDS PT
Product Brochure

1. Why use alternatives to EtBr?
Ethidium bromide (EtBr) is probably the most well-known dye used for visualizing DNA. Because of its chemical structure, it intercalates into the DNA strands forming fluorescent complexes that can be visualized under UV light. But Ethidium bromide is a potential carcinogen and mutagenic agent, so it must be handled with very special care and usually requires specific and exclusive bench space in order not to contaminate the entire laboratory. Because of all the health risks involved while working with EtBr, several alternative DNA stains have been developed during the recent years. Those alternatives are much less dangerous to work with and show a very good staining performance.
NZYTech is proud to announce a portfolio of two alternative nucleic acid stains: GreenSafe Premium and GreenSafe Direct Load. Their sensitive is similar to EtBr and agarose gel electrophoresis can be performed in the exact same way as with EtBr.

Get a Free Discount Coupon if your paper refers NZYTech Products!

If NZYTech’s products are mentioned in a paper published by your lab, you will be rewarded with a Free Discount Coupon of 50 € to use in any order. Please note that the final paper/page proof (as PDF files) or a downloadable link must be provided so NZYTech can confirm that your submission is valid for this promotion.

See more details here.

 

Featured citations

Amplicons with 700 bp were visualized under UV illumination after electrophoresis migration on a 1.5% agarose gel stained with Greensafe premium (Nzytech, Portugal).
Maia, C., Parreira, R., Cristóvão, J. M., Afonso, M. O., Campino, L. (2015). Exploring the utility of phylogenetic analysis of cytochrome oxidase gene subunit I as a complementary tool to classical taxonomical identification of phlebotomine sand fly species (Diptera, Psychodidae) from southern Europe. Acta Tropica 144: 1–8

 

Amplified products were analysed by electrophoresis using 0.5X Tris-Borate-EDTA (TBE) buffer in a 2 % agarose gel stained with GreenSafe (NZYTech) and visualized by transillumination under UV.
Mottola, C., Semedo-Lemsaddek, Mendes, J. J., Melo-Cristino, J., Tavares, L., Cavaco-Silva, P., Oliveira, M. (2016). Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci. Journal of Biomedical Science 8:23:33

 

Both amplicons were visualized under UV illumination after their resolution by conventional electrophoresis on 1.5% agarose gels stained with Greensafe premium® (Nzytech, Portugal), using a 100 bp DNA ladder as a molecular weight marker.
Maia, C., Parreira, R., Cristóvão, J.M., Freitas, F.B., Afonso, M.O., Campino, L. (2015). Molecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera: Psychodidae) from southern Portugal. Parasites & Vectors, 2015 8:173

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