GreenSafe Premium (MB13201)


1 mL


Description: GreenSafe Premium is a new nucleic acid stain that can be used as a safer alternative to the traditional ethidium bromide for detecting nucleic acids in agarose gels. It is as sensitive as ethidium bromide and can be used exactly in the same way in agarose gel electrophoresis. GreenSafe Premium stain emits green fluorescence when bound to DNA or RNA. It has two secondary fluorescence excitation peaks (≈270 nm and ≈290 nm) and one strong excitation peak at 490 nm. The fluorescence emission is similar to ethidium bromide when bound to DNA at 530 nm. Thus GreenSafe Premium stain is compatible with a wide variety of gel reading instruments.

– Used for detecting dsDNA and RNA
– Non-toxic, non-mutagenic and non-carcinogenic (it is an alternative to the ethidium bromide staining)
– High sensitivity even for small fragments
– Superior signal to noise ratio
– Convenient ready-to-use
– Compatible with a wide variety of gel reading instruments
– No hazardous waste

– Detection of DNA or RNA in agarose gels by pre-staining or post-staining

High sensitivity over a wide range of DNA sizes:

GreenSafe Premium_figure1

1% (w/v) agarose gel stained with GreenSafe Premium


Target molecule: DNA and RNA
Detection method: Fluorescence
Mode of use: Add stain during gel casting or alternatively gels can be post-stained
Disposal: Waste must be disposed in accordance with environmental control regulations
Storage conditions: Store at 2-8 ºC, protected from light
Shipping conditions: Shipped at Blue ice to room temperature

– 1 vial of 1 mL

Product Brochure
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN071
Certificate of Analysis YO051
Certificate of Analysis NM011

1. Why use alternatives to EtBr?
Ethidium bromide (EtBr) is probably the most well-known dye used for visualizing DNA. Because of its chemical structure, it intercalates into the DNA strands forming fluorescent complexes that can be visualized under UV light. But Ethidium bromide is a potential carcinogen and mutagenic agent, so it must be handled with very special care and usually requires specific and exclusive bench space in order not to contaminate the entire laboratory. Because of all the health risks involved while working with EtBr, several alternative DNA stains have been developed during the recent years. Those alternatives are much less dangerous to work with and show a very good staining performance.
NZYTech is proud to announce a portfolio of two alternative nucleic acid stains: GreenSafe Premium and GreenSafe Direct Load. Their sensitive is similar to EtBr and agarose gel electrophoresis can be performed in the exact same way as with EtBr.

Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal
Filipe D, Parreira R, Pereira A, Galvão N, Cristovão MJ, Nunes M, Vieira LM, Campino L, Maia, C
Veterinary Parasitology: Regional Studies and Repors, 2021

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
Amorim J P, Bordeira-Carriço R, Gali-Macedo A, Perrod C,
Star Prtocols CellPress, 2020

Giardia duodenalis infection in dogs from the metropolitan area of Lisbon, Portugal:prevalence, genotyping and associated risk factors
Pereira A, Teixeira J, Sousa S, Parreira R, Campino L, Meireles J & Maia Carla
Journal of Parasitic Diseases, 2020

Technical Updates to the Comet Assay In Vivo for Assessing DNA Damage in Zebrafish Embryos from Fresh and Frozen Cell Suspensions
Martins C, Costa PM
Zebrafish, 2020

Phylogenetic insights on Leishmania detected in cats as revealed by nucleotide sequence analysis of multiple genetic markers
Pereira A, Parreira R, Cristóvão JM, Castelli G, Bruno F, Vitale F, Campino L, Maia C
Infection, Genetics & Evolution, 2020

Antibody Response to Toscana Virus and Sandfly Fever Sicilian Virus in Cats Naturally Exposed to Phlebotomine Sand Fly Bites in Portugal
Pereira A, Nazli A, Cristóvão JM, Vilhena H, Martins A, Cachola P, Henriques J, Coimbra M, Catarino A, Lestinova T, Spitzova T, Volf P, Campino L, Charrel R, Maia C
Microorganisms, 2019

Antibody response to Phlebotomus perniciosus saliva in cats naturally exposed to phlebotomine sand flies is positively associated with Leishmania infection
Pereira A, Cristóvão JM, Vilhena H, Martins A, Cachola P, Henriques J, Coimbra M, Catarino A, Lestinova T, Spitzova T, Volf P, Campino L, Maia C
Parasites & Vectors, 2019

Metabolic dynamics of human Sertoli cells are differentially modulated by physiological and pharmacological concentrations of GLP-1
Martins AD, Monteiro MP, Silva BM, Barros A, Sousa M, Carvalho RA, Oliveira PF, Alves MG.
Toxicol Appl Pharmacol, 2019

Tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern Portugal
Pereira A, Parreira R, Cotão AJ, Nunes M, Vieira ML, Azevedo F, Campino L, Maia C
Ticks and Tick-borne Diseases, 2018

Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci
Mottola C, Semedo-Lemsaddek T, Mendes JJ, Melo-Cristino J, Tavares L, Cavaco-Silva P, Oliveira M
Journal of Biomedical Science, 2016

Molecular detection of tick-borne bacteria and protozoa in cervids and wild boars from Portugal
Pereira A, Parreira R, Nunes M, Casadinho A, Vieira ML, Campino L, Maia C
Parasites & Vectors, 2016

Estrogenic regulation of bicarbonate transporters from SLC4 family in rat Sertoli cells
Bernardino RL, Martins AD, Jesus TT, Sá R, Sousa M, Alves MG, Oliveira PF
Mol Cell Biochem, 2015

Exploring the utility of phylogenetic analysis of cytochrome oxidase gene subunit I as a complementary tool to classical taxonomical identification of phlebotomine sand fly species (Diptera, Psychodidae) from southern Europe
Maia C, Parreira R, Cristóvão JM, Afonso MO, Campino L
Acta Tropica, 2015

Molecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera: Psychodidae) from southern Portugal
Maia C, Parreira R, Cristóvão JM, Freitas FB, Afonso MO, Campino L
Parasites & Vectors, 2015

Bacterial and protozoal agents of canine vector-borne diseases in the blood of domestic and stray dogs from southern Portugal
Maia C, Almeida B, Coimbra M, Fernandes MC, Cristóvão JM, Ramos C, Martins Â, Martinho F, Silva P, Neves N, Nunes M, Vieira ML, Cardoso L, Campino L
Parasites & Vectors, 2015

Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications.
Sousa Â, Pereira P, Sousa F, Queiroz JA
Journal of Chromatography A, 2014

Purification of influenza deoxyribonucleic acid-based vaccine using agmatine monolith
Bicho D, Caramelo-Nunes C, Sousa A, Sousa F, Queiroz JA, Tomaz CT
J Chromatogr B Analyt Technol Biomed Life Sci, 2016




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