One-step NZY RT-qPCR Green kit, ROX plus (MB341)

Refrigerated package

101,00458,00

Description: One-step NZY RT-qPCR Green kit, ROX plus is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZY qPCR Green master mix suitable for standard thermal cycling protocols, provided as a 2× reaction mixture, which contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit delivers sensitivity coupled with highly reproducible RT-qPCR protocols on the most common real-time PCR apparatus from either total RNA or mRNA. It is optimized for intercalating green dye detection on different instruments. The latest developments in PCR enhancers have been incorporated in the One-step NZY RT-qPCR Green kit, ROX plus, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results.

Features:
– Easy reaction assembly
– High yields in cDNA synthesis
– Balanced mixture of enzymes (includes RNase inhibitor) with an optimized buffer
– Compatible with instruments that measure the passive reference signal

Components:
– One-step NZY qPCR Green master mix (2x), ROX plus
– NZYRT mix
– DEPC-treated H2O

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN121
Certificate of Analysis ZN111
Certificate of Analysis YO071
Certificate of Analysis YL111
Certificate of Analysis NM111
Certificate of Analysis NM043
Certificate of Analysis NM042
Certificate of Analysis NM041

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