Description: One-step NZY RT-qPCR Green kit, ROX is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZY qPCR Green master mix suitable for standard thermal cycling protocols, provided as a 2× reaction mixture, which contains all components necessary for real-time PCR, including a green intercalating dye, dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit delivers sensitivity coupled with highly reproducible RT-qPCR protocols on the most common real-time PCR apparatus, from either total RNA or mRNA. It is optimized for intercalating green dye detection on different instruments. The latest developments in PCR enhancers have been incorporated in the One-step NZY RT-qPCR Green kit, ROX, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results.
– Easy reaction assembly
– High yields in cDNA synthesis
– Balanced mixture of enzymes (includes RNase inhibitor) with an optimized buffer
– Compatible with instruments that measure the passive reference signal
– One-step NZY qPCR Green master mix (2x), ROX
– NZYRT mix
– DEPC-treated H2O