Description: One-step NZY RT-qPCR Probe kit is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZY qPCR Probe master mix suitable for standard thermal cycling protocols, provided as a 2× reaction mixture, which contains all components necessary for real-time PCR, including dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit delivers sensitivity coupled with highly reproducible RT-qPCR protocols on the most common real-time PCR apparatus, from either total RNA or mRNA. It was developed for probe-detection technology, including molecular beacons. The latest developments in PCR enhancers have been incorporated in the One-step NZY RT-qPCR Probe kit, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results.
– Accurate quantification
– Developed for probe-detection technology
– Balanced mixture of enzymes (includes RNase inhibitor) with an optimized buffer
– Does not contain any passive reference dye (compatible with real-time PCR instruments that do not require a passive reference signal)
– One-step NZY qPCR Probe master mix (2x)
– NZYRT mix
– DEPC-treated H2O