One-step NZY RT-qPCR Probe kit (MB349)

Refrigerated package

101,00458,00

Description: One-step NZY RT-qPCR Probe kit is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZY qPCR Probe master mix suitable for standard thermal cycling protocols, provided as a 2× reaction mixture, which contains all components necessary for real-time PCR, including dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit delivers sensitivity coupled with highly reproducible RT-qPCR protocols on the most common real-time PCR apparatus, from either total RNA or mRNA. It was developed for probe-detection technology, including molecular beacons. The latest developments in PCR enhancers have been incorporated in the One-step NZY RT-qPCR Probe kit, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results.

Features:
– Accurate quantification
– Developed for probe-detection technology
– Balanced mixture of enzymes (includes RNase inhibitor) with an optimized buffer
– Does not contain any passive reference dye (compatible with real-time PCR instruments that do not require a passive reference signal)

Components:
– One-step NZY qPCR Probe master mix (2x)
– NZYRT mix
– DEPC-treated H2O

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN091
Certificate of Analysis ZN081
Certificate of Analysis NM122
Certificate of Analysis NM121
Certificate of Analysis NM031
Certificate of Analysis NM022
Certificate of Analysis NM021

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