One-step NZYSpeedy RT-qPCR Probe kit (MB352)

Refrigerated package

101,00458,00

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Description: One-step NZYSpeedy RT-qPCR Probe kit is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The kit includes a One-step NZYSpeedy qPCR Probe master mix developed for fast PCR, provided as a 2× reaction mixture, which contains all components necessary for real-time PCR, including dNTPs, stabilizers and enhancers. In addition, a separate NZYRT mix that comprises a balanced mixture of both Reverse transcriptase and Ribonuclease Inhibitor is also provided. This kit enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA. It was developed for probe-detection technology, including molecular beacons. The latest developments in PCR enhancers have been incorporated in the One-step NZYSpeedy RT-qPCR Probe kit, including buffer chemistry and a polymerase with hot-start-like activity. This optimized mixture together with the NZYRT mix results in highly sensitive and successful results.

Features:
– Rapid
– Ultra-sensitive
– Developed for probe-detection technology
– Unique buffer chemistry and balanced mixture of enzymes (includes RNase inhibitor)
– Does not contain any passive reference dye (compatible with real-time PCR instruments that do not require a passive reference signal)

Components:
– One-step NZYSpeedy qPCR Probe master mix (2x)
– NZYRT mix
– DEPC-treated H2O

Product Brochure EN
Safety Information EN
MSDS PT
MSDS EN
Informacao de Seguranca PT
Certificate of Analysis ZN041
Certificate of Analysis NM122
Certificate of Analysis NM121
Certificate of Analysis NM031
Certificate of Analysis NM022
Certificate of Analysis NM021

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