Description: NZYSpeedy One-step RT-qPCR Probe Master Mix is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The master mix, formulated as a 2× reaction mixture, contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA, and it was specifically developed for probe-detection technology. The latest developments in PCR enhancers have been incorporated in the NZYSpeedy One-step RT-qPCR Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX. For qPCR instruments that require this reference dye, please add ROX (Cat. No. MB406) according to the table shown in the product brochure (ROX not provided).
– Developed for probe-detection technology
– Unique buffer chemistry and balanced mixture of enzymes (includes RNase inhibitor)
– Does not contain any passive reference dye (compatible with real-time PCR instruments that do not require a passive reference signal)
– NZYSpeedy One-step qPCR Probe Master Mix (2x)