Description: NZYSpeedy One-step RT-qPCR Probe Master Mix is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The master mix, formulated as a 2× reaction mixture, contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA, and it was specifically developed for probe-detection technology. The latest developments in PCR enhancers have been incorporated in the NZYSpeedy One-step RT-qPCR Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX. For qPCR instruments that require this reference dye, we indicate a concentration range for your assay according to the table shown in the product brochure (ROX not provided).
– Developed for probe-detection technology
– Unique buffer chemistry and balanced mixture of enzymes (includes RNase inhibitor)
– Does not contain any passive reference dye (compatible with real-time PCR instruments that do not require a passive reference signal)
– NZYSpeedy One-step qPCR Probe Master Mix (2x)