Lyo NZYSpeedy One-step RT-qPCR Probe Master Mix (2x) (MB41301)


1.5 ml (150 x 20 µL)


Get Any 4, Pay 3 - October 2021
We offer the cheapest product. Not valid for services.

Description: Lyo NZYSpeedy One-step RT-qPCR Probe Master Mix is a freeze-dried reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimize the risk of errors and contaminations. The master mix, formulated as a 2× reaction mixture, is provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. After reconstitution, the mixture contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA, and it was specifically developed for probe-detection technology. The latest developments in PCR enhancers have been incorporated in the Lyo NZYSpeedy One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX. For qPCR instruments that require this reference dye, there is the option to purchase ROX reference dye (Cat. No. MB406).

– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Convenient setup: RT and qPCR in a single step
– Rapid and sensitive
– Includes RNase Inhibitor
– Does not include a passive reference dye

– Lyo NZYSpeedy One-step qPCR Probe Master Mix (2x)
– RT-qPCR master mix reconstitution buffer

– Real-time RT-qPCR (one-step protocol)
– Developed for probe-detection technology

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis NM101

Color-Coding of Microchip RT-PCR Test System for SARS-CoV-2 Detection
Rajwant K. Gill, Sikander S. Gill, Irina Gelimson, Maxim Slyadnev, Gabriel Martinez, Michelle Gaines, Ryan Nunley, Timea Majoros
Journal of Biosciences and Medicines, 2021

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