NZYSupreme One-step RT-qPCR Probe Master Mix (2x) (MB414)


Description: NZYSupreme One-step RT-qPCR Probe Master Mix is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimizes the risk of errors and contaminations. The master mix, formulated as a 2× reaction mixture, contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology. This master mix does not contain ROX. For qPCR instruments that require this reference dye, we indicate a concentration range for your assay according to the table shown in the product brochure (ROX not provided).

– Ultra-sensitive: detects low-copy number targets (8 copies)
– Dual hot-start mode
– Includes RNase Inhibitor
– Suitable for Multiplexing
– Convenient setup: RT and qPCR in a single step
– Does not include a passive reference dye

– NZYSupreme One-step qPCR Probe Master Mix (2x)

– Real-time RT-qPCR (one-step protocol)
– Developed for probe-detection technology

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN122
Certificate of Analysis ZN112
Certificate of Analysis ZN051
Certificate of Analysis ZN023
Certificate of Analysis YO051
Certificate of Analysis YO031
Certificate of Analysis NM111
Certificate of Analysis NM106
Certificate of Analysis NM105
Certificate of Analysis NM104
Certificate of Analysis NM103
Certificate of Analysis NM102
Certificate of Analysis NM101

OmniSARS2: A Highly Sensitive and Specific RT-qPCR-Based COVID-19 Diagnostic Method Designed to Withstand SARS-CoV-2 Lineage Evolution
Carvalho-Correia E, Calçada C, Branca F, Estévez-Gómez N, Chiara L, Varela N, Gallego-García P, Posada D, Sousa H, Sousa J, Veiga MI, Osório NS
Biomedicines, 2021

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