Lyo NZYSupreme One-step RT-qPCR Probe Master Mix (2x) (MB41501)

219,00

1.5 ml (150 x 20 µL)

219,00

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Description: Lyo NZYSupreme One-step RT-qPCR Probe Master Mix is a freeze-dried reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimize the risk of errors and contaminations. The master mix, formulated as a 2× reaction mixture, is provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. After reconstitution, the mixture contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology. For qPCR instruments that require this reference dye, there is the option to purchase ROX reference dye (Cat. No. MB406)

Features:
– Eco-friendly room temperature shipment
– Stable at room temperature for 1 month
– Ultra-sensitive: detects low-copy number targets (8 copies)
– Dual hot-start mode
– Includes RNase Inhibitor
– Convenient setup: RT and qPCR in a single step
– Does not include a passive reference dye

Components:
– Lyo NZYSupreme One-step qPCR Probe Master Mix (2x)
– RT-qPCR master mix reconstitution buffer

Applications:
– Real-time RT-qPCR (one-step protocol)
– Developed for probe-detection technology

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN081
Certificate of Analysis ZN031
Certificate of Analysis NM101

Color-Coding of Microchip RT-PCR Test System for SARS-CoV-2 Detection
Rajwant K. Gill, Sikander S. Gill, Irina Gelimson, Maxim Slyadnev, Gabriel Martinez, Michelle Gaines, Ryan Nunley, Timea Majoros
Journal of Biosciences and Medicines, 2021

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