NcoI (MB066)

Refrigerated package


Description: NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by dam methylation, dcm methylation or CpG methylation. NZYTech’s NcoI is an enzyme that acts at 37 °C.

Isoschizomers: Bsp19I

Concentration: 10000 U/mL

Unit definition: One unit is defined as the amount of this enzyme required to digest completely 1 μg of plasmid DNA in 50 μL of the reaction mixture at 37 °C for one hour.

Incubation temperature: 37 °C for one hour

Heat inactivation: 65 °C for 20 minutes

Reaction Buffer: NZYTech provides NcoI with a specific buffer, NZYBuffer B, and a 0.1% BSA solution. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYTech Buffers:

NZYBuffer A: 20%
NZYBuffer B: 60%*
NZYBuffer C: (60)%
NZYBuffer U: 100%

() weak star activity is detected
* 100% activity is obtained by addition of 0.01% BSA (final concentration)

Product Brochure EN
Certificate of Analysis ZN011
Certificate of Analysis YO051
Certificate of Analysis YO041
Certificate of Analysis YL101
Certificate of Analysis NM121
Certificate of Analysis 15031

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