Description: EcoRI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the EcoRI gene from Escherichia coli RY 13. EcoRI activity is blocked by some combinations of overlapping CpG methylation. NZYTech’s EcoRI is an enzyme that acts at 37 °C.
Concentration: 10000 U/mL
Unit definition: One unit is defined as the amount of this enzyme required to digest completely 1 μg of plasmid DNA in 50 μL of the reaction mixture at 37 °C for one hour.
Incubation temperature: 37 °C for one hour
Heat inactivation: 65 °C for 20 minutes
Reaction Buffer: NZYTech provides EcoRI with a specific buffer, termed NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYTech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (120)%
NZYBuffer C: (80)%
NZYBuffer U: 100%
() weak star activity is detected