EcoRI (MB067)

Refrigerated package


Description: EcoRI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the EcoRI gene from Escherichia coli RY 13. EcoRI activity is blocked by some combinations of overlapping CpG methylation. NZYTech’s EcoRI is an enzyme that acts at 37 °C.

Isoschizomers: FunII

Concentration: 10000 U/mL

Unit definition: One unit is defined as the amount of this enzyme required to digest completely 1 μg of plasmid DNA in 50 μL of the reaction mixture at 37 °C for one hour.

Incubation temperature: 37 °C for one hour

Heat inactivation: 65 °C for 20 minutes

Reaction Buffer: NZYTech provides EcoRI with a specific buffer, termed NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYTech Buffers:
NZYBuffer A: 100%
NZYBuffer B: (120)%
NZYBuffer C: (80)%
NZYBuffer U: 100%

() weak star activity is detected

Product Brochure EN
Certificate of Analysis ZN061
Certificate of Analysis YO061
Certificate of Analysis YO051
Certificate of Analysis YL121
Certificate of Analysis NM081
Certificate of Analysis NM061

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