Description: CciNI (NotI) is a Restriction Endonuclease purified from an Escherichia coli strain that carries the CciNI gene from Curtobacterium citreus N. CciNI (NotI) activity is blocked by CG methylation. NZYTech’s CciNI (NotI) is an enzyme that acts at 37 °C.
Concentration: 5 U/μL
Unit definition: One unit is defined as the amount of this enzyme required to digest completely 1 μg of plasmid DNA in 50 μL of the reaction mixture at 37 °C for one hour.
Incubation temperature: 37 °C for one hour
Heat inactivation: 65 °C for 20 minutes
Reaction Buffer: NZYTech provides CciNI (NotI) with a specific buffer, NZYBuffer A. For double digestions please use 10x NZYBuffer U (MB110, not provided).
Activity in NZYTech Buffers:
NZYBuffer A: 100%
NZYBuffer B: 80%
NZYBuffer C: 60%
NZYBuffer U: 100%