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DpnII

Storage Conditions:
-30 °C to -15 °C
DpnII is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnII gene from Diplococcus pneumoniae. DpnII activity is blocked by Dam methylation. NZYtech’s DpnII is an enzyme that acts at 37 °C.
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Ready to Ship
Dry Ice
From €46.00
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. DpnII is a Restriction Endonuclease purified from an Escherichia coli strain that carries the DpnII gene from Diplococcus pneumoniae. DpnII activity is blocked by Dam methylation. The optimum reaction temperature is at 37 °C.

Concentration: 10000 U/mL

Reaction Buffer: NZYtech provides DpnII with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYtech Buffers:
NZYBuffer A: 80%
NZYBuffer B: 80%
NZYBuffer C: 100%
NZYBuffer U: 100%

Minimize exposure of enzyme to temperatures higher than -20 °C.
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers BfuCI, Bsp143I, BssMI, BstKTI, BstMBI, Kzo9I, MboI, NdeII, Sau3AI
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers BfuCI, Bsp143I, BssMI, BstKTI, BstMBI, Kzo9I, MboI, NdeII, Sau3AI
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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