Description: NZYTech’s Speedy restriction enzymes are a new generation of DNA modifying enzymes developed for rapid DNA digestion. All Speedy enzymes are 100% active in the NZYSpeedyBuffers and are able to digest DNA in 5-15 minutes. NZYSpeedyBuffers are universal and enable any combination of NZY Speedy restriction enzymes to work simultaneously. It’s ideal for double or multiple digestions, eliminating the need for sequential digestions. NZYSpeedyBuffer Orange also allows ready gel load after digestion. Speedy MboI activity is blocked by dam methylation and impaired by overlapping CpG methylation. NZYTech’s Speedy MboI is a fast enzyme that performs its reaction usually in 5-15 minutes at 37 °C.
Concentration: One μL of enzyme can completely digest up to 1 μg of DNA in 5-15 min.
Reagents supplied with the enzyme:
– 10× NZYSpeedyBuffer Colourless
– 10× NZYSpeedyBuffer Orange
Incubation temperature: 37 °C for 5-15 minutes.
Heat inactivation: 65 °C for 20 minutes.