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NZY First-Strand cDNA Synthesis Kit

Storage Conditions:
-85 °C to -15 °C
NZY First-Strand cDNA Synthesis Kit is a convenient and reliable system that includes all the necessary components to synthesize first-strand cDNA. The kit allows reaction at high temperatures, which are benefic when starting from difficult and complex RNA.
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Dry Ice
From €196.00
NZY First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H minus) and the NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA:RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA.
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Dual-primer: oligo(dT) + random hexamers, Fast, High yield, Thermostable
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 50 °C
Protocol time Approx. 60 min
– NZYRT Enzyme Mix
– NZYRT 2x Master Mix
– NZY RNase H (E. coli)
– DEPC-treated H2O
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Dual-primer: oligo(dT) + random hexamers, Fast, High yield, Thermostable
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 50 °C
Protocol time Approx. 60 min
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Dual-primer: oligo(dT) + random hexamers, Fast, High yield, Thermostable
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 50 °C
Protocol time Approx. 60 min

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