Lyo NZYRibonuclease Inhibitor (MB41101)

1.799,00

100000 U

1.799,00

Description: Lyo NZY Ribonuclease Inhibitor is a freeze-dried enzyme preparation provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. The enzyme is subjected to rigorous purification protocols to ensure optimal performance. Lyo NZY Ribonuclease Inhibitor inhibits the activity of ribonucleases (RNases; EC 3.1) of the pancreatic type, such as RNase A, RNase B and RNase C, by binding them noncovalently in a 1:1 ratio. NZY Ribonuclease Inhibitor is useful in any application where RNase contamination is a potential problem. For instance, it can be used to protect template RNA in cDNA synthesis reactions, RT-PCR or in vitro transcription/translation, as well as to protect viral RNA during in vitro replication. In addition, it will inhibit RNases during RNA isolation and purification and during RNase-free antibodies preparation. NZY Ribonuclease Inhibitor is not active against RNase 1, RNase T1, RNase T2, S1 nuclease and RNase H.

Features:
– Eco-friendly room temperature shipment
– Inhibitor of RNase A, B and C
– Protects RNA at temperatures up to 55 ºC

Applications:
Protects RNA from degradation in the following situations:
– cDNA synthesis by reverse transcription
– in vitro transcription/ translation
– RNA amplification
– RNA purification and storage

Specifications:

Optimal reaction temperature: 50 ºC
Denaturing conditions:NZY Ribonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate this protein
Liquid format available:NZYRibonuclease Inhibitor (MB084)
Storage conditions:Store at -20 ºC
Shipping conditions:Shipped on dry ice to room temperature

Components:
– Lyo Ribonuclease Inhibitor (40 U/µL)
– Lyo NZY-RI resuspension buffer

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN012
Certificate of Analysis ZN011

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