Description: T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of highly specific activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5’→3’ synthesis of RNA from ribonucleoside triphosphates on single or double stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis.
Features:
– Quick and easy production of RNA transcripts from DNA template containing a RNA polymerase promotor
– Convenient and reliable
– Incorporates modified nucleotides as substrates for RNA synthesis
Applications:
– Synthesis of RNA transcripts, biologically active mRNA and antisense RNA
– Production of RNA for in vitro translation and gene expression studies
– Generation of labelled RNA probes
Specifications:
| Specificity: | Highly promoter-specific. Only transcribes DNA cloned downstream of T7 promoter |
| Optimal reaction temperature: | 37 ºC |
| Denaturing conditions: | T7 RNA polymerase can be inactivated by adding 20 mM EDTA (pH 8.0) final concentration or by heating at 70 °C for 10 min |
| Storage conditions: | Store at -20 ºC |
| Shipping conditions: | Shipped at 4 ºC to dry ice |
Components:
– T7 RNA Polymerase (20 U/μL or 200 U/μL)
– Reaction Buffer (10x)
