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T7 RNA Polymerase
T7 RNA Polymerase is a DNA-dependent RNA polymerase with high promoter-specificity. The enzyme only transcribes DNA downstream of a T7 promoter.
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Dry Ice
T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of high specific-activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5’→3’ synthesis of RNA from ribonucleoside triphosphates on single or double-stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis.
Shipping Conditions | Dry Ice |
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Storage Conditions | -85 °C to -15 °C |
Application | In vitro RNA synthesis |
Format | Enzyme/Protein, Liquid |
Product Category | RNA Synthesis |
Reaction Temperature | 37 °C |
Heat Inactivation | 70 °C |
Protocol time | Approx. 120 min |
– T7 RNA Polymerase (20 U/μL or 200 U/μL)
– Reaction Buffer (10x)
– Reaction Buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | In vitro RNA synthesis |
Format | Enzyme/Protein, Liquid |
Product Category | RNA Synthesis |
Reaction Temperature | 37 °C |
Heat Inactivation | 70 °C |
Protocol time | Approx. 120 min |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | In vitro RNA synthesis |
Format | Enzyme/Protein, Liquid |
Product Category | RNA Synthesis |
Reaction Temperature | 37 °C |
Heat Inactivation | 70 °C |
Protocol time | Approx. 120 min |
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