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NZY RNase H (E. coli)
NZY RNase H is a key enzyme in the synthesis of cDNA. It specifically hydrolyses RNA in RNA:cDNA complexes, thus improving sensitivity. The enzyme does not degrade single and double-stranded DNA or unhybridized RNA.
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Dry Ice
NZY RNase H is a recombinant endoribonuclease purified from an Escherichia coli strain that over-expresses the cloned RNase H gene (rnh). The enzyme specifically hydrolyses the phosphodiester bonds of RNA which is hybridized to DNA to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. RNase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as cDNA-RNA hybrids in the amplification reaction may prevent binding of the amplification primers. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets.
Shipping Conditions | Dry Ice |
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Storage Conditions | -85 °C to -15 °C |
Application | cDNA synthesis, RT-PCR, RT-qPCR |
Format | Enzyme/Protein, Liquid |
Product Category | RNases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
– NZY RNase H (E. coli) (5 U/μL)
– Reaction buffer (10x)
– Reaction buffer (10x)
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | cDNA synthesis, RT-PCR, RT-qPCR |
Format | Enzyme/Protein, Liquid |
Product Category | RNases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85 °C to -15 °C |
Application | cDNA synthesis, RT-PCR, RT-qPCR |
Format | Enzyme/Protein, Liquid |
Product Category | RNases |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
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