Lyo NZYRibonuclease Inhibitor (no DTT) (MB41201)

1.799,00

100000 U

1.799,00

Description: Lyo NZY Ribonuclease Inhibitor (no DTT) is a freeze-dried enzyme preparation provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. The enzyme is subjected to rigorous purification protocols and displays improved resistance to oxidation, which allows it to be stable at DTT concentrations lower than 1 mM. This property makes this ribonuclease inhibitor ideal for reactions that do not tolerate higher levels of DTT (>1 mM) and were RNases (EC 3.1) contamination is a potential problem, such as in real-time RT-PCR assays. Lyo NZY Ribonuclease Inhibitor (no DTT) inhibits the activity of the omnipresent RNases of the pancreatic type, such as RNase A, RNase B and RNase C, by binding them noncovalently in a 1:1 ratio. This enzyme is not active against RNase 1, RNase T1, RNase T2, S1 nuclease and RNase H.

Features:
– Eco-friendly room temperature shipment
– High oxidation resistance (which can be helpful in situations where DTT cannot be used)
– Inhibitor of RNase A, B and C
– Protects RNA at temperatures up to 55 ºC

Applications:
Protects RNA from degradation in the following situations:
– cDNA synthesis by reverse transcription
– RT-PCR
-in vitro transcription/ translation
– RNA labelling
– RNA isolation and purification

Specifications:

Optimal reaction temperature: 50 ºC
Denaturing conditions:NZY Ribonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate this protein
Lyo format available:NZY Ribonuclease Inhibitor (no DTT) (MB410)
Storage conditions:Store at -20 ºC
Shipping conditions:Shipped on dry ice to room temperature

 

Components:
– Lyo Ribonuclease Inhibitor (no DTT) (40 U/µL)
– Lyo NZY-RI-noDTT resuspension buffer

Product Brochure EN
Certificate of Analysis NM121

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