Random hexamer mix (MB12901)

Refrigerated package

49,00

25 μg (500 μL)

49,00

Description: Random hexamer mix includes oligonucleotides representing all possible hexamer sequences. Random hexamers are commonly used for priming single-stranded DNA or RNA for extension by DNA polymerase or reverse transcriptase, typically when the template is difficult to copy in its entirely. During cDNA synthesis, random hexamer will perform random priming throughout the entire length of the RNA to generate a cDNA pool containing various lengths of cDNA. With this method, all RNAs present in a population constitute templates for cDNA synthesis experiment.

Features:
– Ultra-pure grade
– DNase/ RNase free

Applications:
– Priming ssDNA or RNA for extension by DNA polymerase or Reverse transcriptases

Specifications:
– Primer sequence: 5’- d (NNNNNN)-3’, where N = G, A, T or C
– Storage conditions: Store at -20 ºC
– Shipping conditions: Shipped on dry ice

Components:
– Random hexamer mix (500 µL at 50 ng/µL)

MSDS EN
MSDS PT
Product Brochure EN

random_hexamer_priming_method
Random hexamers randomly bind throughout the entire length of all RNA (tRNA, rRNA, and mRNA)

Estrogenic regulation of bicarbonate transporters from SLC4 family in rat Sertoli cells
Bernardino RL, Martins AD, Jesus TT, Sá R, Sousa M, Alves MG, Oliveira PF
Mol Cell Biochem, 2015

Metabolic fingerprints in testicular biopsies from type 1 diabetic patients
Alves MG, Martins AD, Moreira PI, Carvalho RA, Sousa M, Barros A, Silva J, Pinto S, Simões T, Oliveira PF
Cell Tissue Res, 2015

Leptin modulates human Sertoli cells acetate production and glycolytic profile: a novel mechanism of obesity-induced male infertility?
Martins AD, Moreira AC, Sá R, Monteiro MP, Sousa M, Carvalho RA, Silva BM, Oliveira PF, Alves MG
Biochem Biophys Act, 2015

Aquaporin-4 as a molecular partner of cystic fibrosis transmembrane conductance regulator in rat Sertoli cells
Jesus TT, Bernardino RL, Martins AD, Sá R, Sousa M, Alves MG, Oliveira PF
Biochem Biophys Res Commun, 2014

Aquaporin-9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator
Jesus TT, Bernardino RL, Martins AD, Sá R, Sousa M, Alves MG, Oliveira PF.
IUBMB Life, 2014