NZYRibonuclease Inhibitor (MB084)

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29,00199,00

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Description: NZY Ribonuclease Inhibitor is a recombinant protein purified from Escherichia coli that inhibits the activity of ribonucleases (RNases; EC 3.1) of the pancreatic type. The protein is useful in any application where eukaryotic RNase contamination is a potential problem. It is not active against RNase 1, RNase T1, RNase T2, S1 nuclease and RNase H. In first-strand cDNA synthesis experiments, 40 units of protein in a 20 µL reaction mixture protect the template RNA, improve total cDNA yields and increase the percentage of full length cDNA. The presence of NZY Ribonuclease Inhibitor does not affect the use of RNase H after first-strand cDNA synthesis. NZY Ribonuclease Inhibitor requires 0.5 to 1 mM DTT (not provided) in the reaction system to maintain activity. The storage buffer of this protein contains 8 mM DTT, but additional DTT is required if volume of the inhibitor in the reaction mixture is less than 1/8 of the total volume.

Features: 
– Inhibitor of RNase A, B and C
– Protects RNA at temperatures up to 55 ºC

Applications:
Protects RNA from degradation in the following situations:
– cDNA synthesis by reverse transcription
– in vitro transcription/ translation
– RNA amplification
– RNA purification and storage

Specifications:

Optimal reaction temperature:  50 ºC
Denaturing conditions: NZY Ribonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate this protein
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped on dry ice

Components:
– Ribonuclease Inhibitor (40 U/µL or 20 U/µL)

MSDS EN
MSDS PT
Product Brochure EN

1. NZYRibonuclease Inhibitor is not showing inhibition activity. What is the reason?
This may result from several factors, such as:
a) Type of RNases present in the reaction. Ribonucleases for which NZYRibonuclease Inhibitor has no activity may be present as contaminants in the reaction. The enzyme inhibits the activity of ribonucleases (RNases; EC 3.1) of the pancreatic type, such as RNase A, RNase B and RNase C.
b) Concentration of DTT. Check if enough amount of DTT is present in the reaction. NZYRibonuclease Inhibitor requires 0.5 to 1 mM DTT to maintain activity.
c) Denaturing conditions. NZYRibonuclease Inhibitor is inhibited by common denaturants such as SDS, urea and all oxidizing reagents. Temperatures above 65 °C also inactivate the inhibitor. There is some residual activity up to 50-55 °C.

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