T7 RNA Polymerase (MB080)

Refrigerated package

50,00

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Description: T7 RNA polymerase is a recombinant enzyme purified from Escherichia coli, for the synthesis of highly specific activity RNA probes, biologically active mRNA and antisense RNA. T7 RNA polymerase is DNA-dependent with strict specificity for its own double-stranded promoter that is not efficiently recognized by SP6 or T3 RNA polymerases. T7 RNA polymerase catalyzes the 5’→3’ synthesis of RNA from ribonucleoside triphosphates on single or double stranded DNA downstream from a T7 promoter. Using circular plasmid DNA as a template will result in heterogeneous transcripts of multiple lengths. T7 RNA polymerase accepts modified nucleotides as substrates for RNA synthesis.

Features:
– Quick and easy production of RNA transcripts from DNA template containing a RNA polymerase promotor
– Convenient and reliable
– Incorporates modified nucleotides as substrates for RNA synthesis

Applications:

– Synthesis of RNA transcripts, biologically active mRNA and antisense RNA
– Production of RNA for in vitro translation and gene expression studies
– Generation of labelled RNA probes

Specifications:

Specificity:Highly promoter-specific. Only transcribes DNA cloned downstream of T7 promoter
Optimal reaction temperature:37 ºC
Denaturing conditions: T7 RNA polymerase can be inactivated by adding 20 mM EDTA (pH 8.0) final concentration or by heating at 70 °C for 10 min
Storage conditions:Store at -20 ºC
Shipping conditions:Shipped at 4 ºC to dry ice

Components:
– T7 RNA Polymerase (20 U/μL or 200 U/μL)
– Reaction Buffer (10x)

Product Brochure EN
MSDS PT
MSDS EN

T7 RNA PolymeraseAgarose gel analysis of RNA transcripts produced using T7 RNA polymerase. The RNA transcripts were generated from indicated amounts of DNA template using 20 U (lanes 1 and 3) or 200 U (lanes 2 and 4) of T7 RNA polymerase. M, NZYDNA Ladder I

 

 

 

 

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