Description: NZYTech provides DNA oligos built to your specifications suitable for several applications, including PCR, cloning, gene detection and sequencing. NZYTech oligos are delivered with our Products Specifications Sheet that includes: sequence, yield in nmol and µg, melting temperature, MW, GC%, purification mode and quality control. NZYTech offers four different synthesis scales: 10 nmol, 40 nmol, 200 nmol and 1000 nmol and Standard and High Purity oligos:
For nonlabeled, standard oligos, up to 40 bases, we guarantee a minimum yield:
The different scales have restrictions regarding the length of the oligo that can be synthesized, as specified in the table below:
* For long oligos it is recommended, for some applications, to perform an HPLC purification
NZYTech offers a broad range of modifications for all types of Real-Time PCR (qPCR) platforms. Please contact NZYTech (oligos@nzytech.com) if you need more information about the modification you would like.
Delivery Specifications:
– NZYTech oligos are shipped dry
– Packed in individual-labeled microcentrifuge tubes (contact us if you need your oligos in 96 wells plate-format)
– Under normal conditions standard oligos are shipped within 4-5 working days
– Shipment of larger orders, purified oligos and labeled oligos will take a few days more
– Please contact NZYTech for information about delivery times and shipment costs
Quotes & Ordering:
To order NZYTech Oligos, please download the Order Template Form and send it as an Excel attachment to oligos@nzytech.com. The order form also includes guidelines for ordering.
Get a Free Discount Coupon if your paper refers NZYTech Products!
If NZYTech’s products are mentioned in a paper published by your lab, you will be rewarded with a Free Discount Coupon of 50 € to use in any order. Please note that the final paper/page proof (as PDF files) or a downloadable link must be provided so NZYTech can confirm that your submission is valid for this promotion.
See more details here.
Featured citations
The 17-mer oligonucleotides 5’-TAMRA-TC ACC *TC GTA CGA CTC-3’ and 3’-BHQ-2-AG TGG GAG CAT GCT GAG-5’ (in which TAMRA is the fluorophore carboxytetramethylrhodamine, BHQ-2 is black hole quencher-2 and * is tetrahydrofuran, a stable abasic site analogue) (35) were custom-made by NZYTech (Lisbon, Portugal).
Guerreiro, P.S., Estácio, S.G., Antunes, F., Fernandes, A.S., Pinheiro, P.F., Costa, J.G., Castro, M., Miranda, J.P., Guedes, R.C., Oliveira, N.G. (2016). Structure-based virtual screening towards the discovery of novel inhibitors of the DNA repair activity of the human apurin ic/apyrimidinic endonuclease 1. Chem Biol Drug Des 88(6):915-925.
Primers and reagents used for PCR amplifications were purchased from NZYTech Lda (Lisbon, Portugal).
Oliveira, M., Tavares, M, Gomes, D., Touret, T., Braz, B. S., Tavares, L., Semedo-Lemsaddek, T. (2016). Virulence traits and antibiotic resistance among enterococci isolated from dogs with periodontal disease. Comparative Immunology, Microbiology and Infectious Diseases 46:27-31
The sequence of the signal peptide derived from the small tetra-heme cytochrome c (STC) from S. oneidensis MR-1 was used to produce a chimeric gene with therJR_0333 gene from T. potens using the primers listed in Table 1 (NZYtech, Portugal)
Costa, N. L., Carlson, H. K., Coates, J. D., Louro, R. O., Paquete, C. M. (2015). Heterologous expression and purification of a multiheme cytochrome from a Gram-positive bacterium capable of performing extracellular respiration. Protein Expression and Purification 111: 48-52
The primer sequences were designed and synthesized by NZYTech and are described in Table 2. All measurements were performed in triplicate. A single melting peak was obtained for each gene analyzed in all samples.
Menino, J. F., Saraiva, M., Gomes-Rezende, J., Sturme, M., Pedrosa, J., et al. (2013) P. brasiliensis Virulence Is Affected by SconC, the Negative Regulator of Inorganic Sulfur Assimilation. PLoS ONE 8(9): e74725
